Exploration of biosurfactant to develop high efficiency and safety vector for gene transfection

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An unsaturated hydrocarbon chain in phospholipid was reported to affect a phase transition and a fusogenic activity after mixing membranes, and consequently to achieve a high DNA transfection efficiency. We previously showed that a biosurfactant mannosylerythritol lipid-A (MEL-A) enhances the gene transfection efficiency of cationic liposomes. Here, we have studied the effects of unsaturated fatty acid ratio of MEL-A on the gene transfer efficiency by cationic liposomes using MEL-A with different unsaturated fatty acid ratios. As a result, we demonstrated that the MEL-A unsaturated fatty acid ratio strongly affects transfection efficiency.