Structural diversity of L-type Ca^<2+> channel in sinoatrial node-the possibility of Cav-Cav interaction-

窦房结L型Ca^<2>通道的结构多样性-Cav-Cav相互作用的可能性-

• 批准号: 21790199
• 负责人: TOYODA Futoshi
• 金额: $ 2.75万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790199/
• 关键词: 生体膜; チャネル; トランスポーター; 能動輸送; 生理学; 生体分子; 細胞・組織; 循環器; イオンチャネル; ペースメーカー

In the present study, we examined the possibility of the functional assembly of Cav1.2 and Cav1.3 subunits to yield the sustained inward Na^+ current (I_<st>), a pacemaker current in sinoatrial node. Heterologous coexpression of Cav1.2 and Cav1.3 in HEK cells resulted in an ensemble of Ca^<2+> currents attributed to each Cav channel. On the other hand, the chimeric channel consisting of repeat I and II from Cav1.2 (or Cav1.3) and repeat III and IV from Cav1.3 (or Cav1.2) elicited a rapidly inactivating Ca^<2+> current but failed to evoke a Na^+ current similar to I_<st>. Taken together, it is not likely that the heteromeric assembly of Cav1.2 and Cav1.3 subunits underlies I_<st> in sinoatrial node.

在本研究中，我们检测了Cav1.2和Cav1.3亚基功能性组装产生持续性内向Na^+电流（I_）的可能性<st>，I_是窦房结的起搏电流。Cav1.2和Cav1.3在HEK细胞中的异源共表达导致了归因于每个Cav通道的Ca^2+电流集合。另一方面，由来自Cav1.2（或Cav1.3）的重复序列I和II以及来自Cav1.3（或Cav1.2）的重复序列III和IV组成的嵌合通道可引起快速失活的Ca^2+电流，但不能引起类似于I的Na^+电流<st>。综上所述，这是不可能的，Cav1.2和Cav1.3亚基的异聚体组装的基础，在窦房结I_<st>。

项目成果

Generation of sodium-permeable CaV1.3 channel : insights into the molecular basis for the sustained inward current in cardiac pacemaker cells

钠渗透性 CaV1.3 通道的产生：深入了解心脏起搏细胞持续内向电流的分子基础

• 发表时间: 2010
• 作者: Toyoda F;Ding WG;Matsuura H.;Toyoda F
• 通讯作者: Toyoda F

NaA^+透過性CaV1.3チャネルの作製-I_<st>の分子基盤の解明を目指して-

NaA^+可渗透CaV1.3通道的制备 - 旨在阐明I_<st>的分子基础 -

• 发表时间: 2009
• 作者: Toyoda F;Ding WG;Matsuura H.;Toyoda F;豊田太・丁維光・松浦博
• 通讯作者: 豊田太・丁維光・松浦博

D85N, a KCNE1 Polymorphism, Is a Disease-Causing Gene Variant in Long QT Syndrome

• DOI: 10.1016/j.jacc.2009.06.005
• 发表时间: 2009-08-25
• 期刊: JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
• 影响因子: 24
• 作者: Nishio, Yukiko;Makiyama, Takeru;Horie, Minoru
• 通讯作者: Horie, Minoru

Characterization of the Rapidly Activating Delayed Rectifier Potassium Current, I Kr, in HL-1 Mouse Atrial Myocytes

• DOI: 10.1007/s00232-010-9257-2
• 发表时间: 2010-06-01
• 期刊: JOURNAL OF MEMBRANE BIOLOGY
• 影响因子: 2.4
• 作者: Toyoda, Futoshi;Ding, Wei-Guang;Matsuura, Hiroshi
• 通讯作者: Matsuura, Hiroshi

Generation of sodium-permeable CaV1.3 channel : insights into the molecular basis for the sustained inward current in cardiac pacemaker cells.

钠渗透性 CaV1.3 通道的生成：深入了解心脏起搏器细胞中持续内向电流的分子基础。

• 发表时间: 2010
• 作者: Toyoda F;Ding WG;Matsuura H.
• 通讯作者: Matsuura H.