Analysis of the role of Rae1 on carcinogenesis and chromosome instability.

核孔复合体蛋白Rae1的异常表达可导致染色体的非整倍体和多极纺锤体。有报道称Rae1是乳腺癌患者的危险因素之一。本研究试图从体内外两方面分析Rae1与乳腺癌的关系。因此，我们成功地建立了Rae1转基因小鼠，以分析Rae1在体内的表达。此外，我们建立了Rae1在昆虫细胞Sf9中的表达并纯化，这对于生化分析是有用的。

Abnormal expression of nuclear pore complex protein Rae1 makes aneuploidy and multi-polar spindles. It has been reported that Rae1 is one of the risk factors in breast cancer patients. In this study, we tried to analyze the relationship between Rae1 and breast cancer in vitro and in vivo. Therefore we successfully generated Rae1 transgenic mice in order to analysis of Rae1 expression in vivo. Moreover, we established Rae1 expression in insect cell Sf9 and purification it, which are useful for biochemical analysis.