Roles of the nectin-afadin system in epithelial-mesenchymal and mesenchymal-epithelial transitions

Nectin-afadin系统在上皮-间质和间质-上皮转化中的作用

• 批准号: 21790319
• 负责人: OGITA Hisakazu
• 金额: $ 2.75万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790319/
• 关键词: ネクチン; アファディン; ネクチン様分子; インテグリン; 増殖因子受容体; 低分子量Gタンパク質; がん; 上皮細胞

The disruption of cell-cell junctions and the increase in cell movement play important roles in the epithelial-mesenchymal transition. On the other hand, the cell-cell junction formation and cell polarization at the interface between neighboring cells are crucial for the mesenchymal-epithelial transition. In this study, nectin-like molecules, which resemble cell adhesion molecules nectins in their molecular structure, afadin, which binds to the cytoplasmic region of nectins, and their related molecules were shown to promote the growth factor-induced cell movement. The underlying mechanisms were also elucidated. The interaction of afadin with another adaptor protein was essential for the cell polarization of epithelial cells.

细胞-细胞连接的破坏和细胞运动的增加在上皮-间质转化中起重要作用。另一方面，细胞间连接的形成和相邻细胞间界面的细胞极化是间质-上皮转变的关键。本研究发现，在分子结构上与细胞粘附分子连接素相似的连接素样分子、结合在连接素细胞质区域的afadin及其相关分子均可促进生长因子诱导的细胞运动。其潜在的机制也被阐明。afadin与另一接头蛋白的相互作用对上皮细胞的细胞极化至关重要。

项目成果

Localization of nectin-free afadin at the leading edge and its involvement in directional cell movement induced by platelet-derived growth factor

• DOI: 10.1242/jcs.048439
• 发表时间: 2009-12-01
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Miyata, Muneaki;Ogita, Hisakazu;Takai, Yoshimi
• 通讯作者: Takai, Yoshimi

Regulation by Afadin of Cyclical Activation and Inactivation of Rap1, Rac1, and RhoA Small G Proteins at Leading Edges of Moving NIH3T3 Cells

• DOI: 10.1074/jbc.m109.016436
• 发表时间: 2009-09-04
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Miyata, Muneaki;Rikitake, Yoshiyuki;Takai, Yoshimi
• 通讯作者: Takai, Yoshimi

ネクチン?アファディン系による細胞の運動と接着制御におけるホスファターゼの役割と作用機構(シンポジウム)

磷酸酶在 nectin-afadin 系统控制细胞运动和粘附中的作用和作用机制（研讨会）

• 发表时间: 2009
• 作者: 扇田久和;高井義美
• 通讯作者: 高井義美

Silencing of ErbB3/ErbB2 Signaling by Immunoglobulin-like Necl-2

• DOI: 10.1074/jbc.m109.025155
• 发表时间: 2009-08-28
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Kawano, Satoshi;Ikeda, Wataru;Takai, Yoshimi
• 通讯作者: Takai, Yoshimi

新しいがん浸潤・転移のメカニズム

癌症侵袭和转移的新机制

• 发表时间: 2010
• 作者: Toshinori Kinoshita;Eigo Ando;Aiko Watanabe;Shin-ichiro Inoue;扇田久和
• 通讯作者: 扇田久和