Study for the mechanism of HIV-1-specific CTL induction in the intestinal mucosa

肠粘膜中HIV-1特异性CTL诱导机制的研究

• 批准号: 21790453
• 负责人: TERAHARA Kazutaka
• 金额: $ 2.75万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790453/
• 关键词: 感染防御; ワクチン; ウイルス; 免疫学; サルモネラ; HIV; 腸管

In this study, we aimed to elucidate the mechanism by which human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTLs) are induced in the intestine. HIV-1 Gag-expressing Salmonella typhimurium aroA- or aroA-invAmutants, which can or not, respectively, penetrate Peyer's patches, were used as oral antigens into mice. However, Gag-specific CTLs were hardly induced in both the systemic and the gut mucosal compartments. Further study should be needed for better understanding of the role of Peyer's patches in the induction of mucosal CTLs.

在这项研究中，我们旨在阐明人类免疫缺陷病毒（HIV）特异性细胞毒性T淋巴细胞（ctl）在肠道中诱导的机制。表达HIV-1 gag的鼠伤寒沙门氏菌aroA-或aroA-突变体，分别能或不能穿透Peyer's补丁，被用作小鼠的口服抗原。然而，gag特异性ctl在全身和肠粘膜室中都很难被诱导。为了更好地了解Peyer's贴片在诱导粘膜ctl中的作用，还需要进一步的研究。

项目成果

Characteristic activation/differentiation phenotype of CD4^<+> T cells and their distinct susceptibility to X4-type and/or R5-type HIV-1 infection in humanized NOD/SCID/Jac3-null mice

人源化 NOD/SCID/Jac3 缺失小鼠中 CD4^< > T 细胞的特征激活/分化表型及其对 X4 型和/或 R5 型 HIV-1 感染的独特易感性

• 发表时间: 2010
• 作者: Terahara K;Ishige M;Mitsuki Y;Shibusawa K;Watanabe S;Okada S;Kobayashi K;Tsunetsugu-Yokota Y
• 通讯作者: Tsunetsugu-Yokota Y

Inhibition of HIV-1 replication by short hairpin RNA is augmented by using lentivirus vector pseudotyped with envelope proteins of wild-type measles virus.

使用野生型麻疹病毒包膜蛋白假型化的慢病毒载体可增强短发夹 RNA 对 HIV-1 复制的抑制作用。

• 发表时间: 2011
• 作者: Shibusawa K;Mitsuki Y;Terahara K;Ishige M;Yanagi Y;Kobayashi K;Tsunetsugu-Yokota Y
• 通讯作者: Tsunetsugu-Yokota Y

Inhibition of HIV-1 replication by envelope-engineered lentivirus vectors : efficient gene delivery using envelope protein of measles virus

包膜工程慢病毒载体抑制 HIV-1 复制：利用麻疹病毒包膜蛋白进行有效基因递送

• 发表时间: 2010
• 作者: Shibusawa K;Mitsuki Y;Terahara K;Ishige M;Yanagi Y;Kobayashi K;Tsunetsugu-Yokota Y
• 通讯作者: Tsunetsugu-Yokota Y

Inhibition of HIV-1 replication by envelope-engineered lentivirus vectors : efficient gene delivery using envelope protein of measles virus.

通过包膜工程慢病毒载体抑制 HIV-1 复制：使用麻疹病毒包膜蛋白进行有效基因递送。

• 发表时间: 2010
• 作者: Shibusawa K;Mitsuki Y;Terahara K;Ishige M;Yanagi Y;Kobayashi K;Tsunetsugu-Yokota Y
• 通讯作者: Tsunetsugu-Yokota Y

Factors crucial for the preferential propagation of R5-tropic HIV-1 in the early phase of HIV-1 infection

HIV-1感染早期R5嗜性HIV-1优先传播的关键因素

• 发表时间: 2010
• 作者: Tsunetsugu-Yokota Y;Terahara K
• 通讯作者: Terahara K