Estimation of DNA degradation and STR success rate by determining the DNA degradation ratio using quantitative PCR

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Quantitative PCR assays using primers that generate two sizes of amplicons from the same region of genomic DNA were used to determine the extent of DNA degradation. The distribution of the extent of DNA degradation and the number of loci detected by STR analysis in blood stains correlated well with that of DNA artificially degraded by DNase I in the presence of Mn^<2+>. Thus, determination of the extent of DNA degradation was helpful for estimating the number of detectable loci. We were able to remove the PCR inhibitory substances that were copurified with DNA from bone sample using the new DNA extraction procedure. Therefore, the degradation ratio appeared to be a useful for predicting the number of loci detected by STR analysis in casework sample.