Study on isolation, characteristic and distribution of pediatric leukemic stem cell using NOG mouse model

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Primary samples from patients with leukemia were successfully engrafted into mice, and those engrafted leukemic cells were able to be serially transplanted into secondary, tertiary recipients. Morphological and FACS analyses revealed as high as >80% blood chimerism and conserved blast phenotypes through serial transplantations. Moreover, extramedullary organs including liver, spleen and kidney showed the leukemic invasion consistent with donor disease. Immunohistological analysis of liver revealed that SDF-1 was detectable only in bile duct epithelial cells. In addition, we demonstrated directly the effect of SDF-1/CXCR4 axis in our model by using the CXCR4 inhibitor both in vivo and in vitro. Our study on the involvement of SDF-1/CXCR4 axis in liver could rink to the novel therapies which target the extramedullary sites in order to perfectly overcome leukemia.