Functional characterization of two new sugar transporter families within the Major Facilitator Superfamily (MFS)

主要促进子超家族 (MFS) 内两个新糖转运蛋白家族的功能表征

• 批准号: 5387959
• 负责人: Privatdozent Dr. Michael Büttner
• 金额: --
• 依托单位: Centre for Organismal Studies (COS)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5387959?language=en
• 关键词: Functional; characterization; two; new; sugar

Our group has almost completely characterized a family of monosaccharide transporters in Arabidopsis (AtSTPs) comprising 14 highly homologous members. For most AtSTPs, we could demonstrate a sink-specific expression and the localisation in the plasmamembrane. Expression in yeast cells showed that most of these transporters accept a broad range of monosaccharide substrates. In addition to the AtSTPs, 6 distinct families of putative sugar transporters can be identified within the Arabidopsis genome. One of these families shows significant homologies to bacterial xylose/H+ symporters, while another family is related to vacuolar transporters and mammalian facilitators. We wish to functionally characterize these two families by investigating the transport properties, the tissue-specific and (sub)cellular localizations as well as the developmental, spatial, and environmental regulation of the individual transporters.

我们的小组已经几乎完全确定了一个家庭的单糖转运蛋白在拟南芥（AtSTPs），包括14个高度同源的成员。对于大多数AtSTPs，我们可以证明一个水槽特异性表达和定位在质膜。在酵母细胞中的表达表明，大多数这些转运蛋白接受广泛的单糖底物。除了AtSTPs外，在拟南芥基因组中还可以鉴定出6个不同的推定糖转运蛋白家族。其中一个家族与细菌木糖/H+共转运蛋白具有显著的同源性，而另一个家族与液泡转运蛋白和哺乳动物易化蛋白有关。我们希望通过研究运输特性，组织特异性和（亚）细胞定位以及个体转运蛋白的发育，空间和环境调节来功能性地表征这两个家族。