Identification of synovial sarcoma stem cell as a new therapeutic target

我们尝试用球培养法鉴定滑膜肉瘤干细胞。茎干性基因Oct3、Nanog和Sox2在球状亚群中表达量较高，其致瘤性也较高。接下来，我们比较了球形和非球形亚群之间的基因表达谱，并确定了干细胞标记的候选基因。利用特异性抗体和细胞分选器对候选基因阳性亚群和阴性亚群进行划分，分析其致瘤性差异。结果表明，候选基因阳性亚群比阴性亚群具有更高的致瘤性。本研究首次检测到滑膜肉瘤干细胞和干细胞标记物，对开发新的滑膜肉瘤治疗方法具有重要意义。

We attempted to identify a synovial sarcoma stem cell with sphere culture. The higher expression of stemness genes, Oct3, Nanog, and Sox2 was observed in the sphere subpopulation, and higher tumorigenicity was also observed in the xeno-plantation made from sphere subpopulation. Next, we compare the gene expression profiles between sphere and non-sphere subpopulation and determined candidate genes of the stem cell marker. Using the specific antibodies and cell sorter, we divided candidate gene positive and negative subpopulation, and analyzed the difference of tumorigenicity. We conformed that candidate gene positive subpopulation possess higher tumorigenicity than negative one.In this study, we first detect synovial sarcoma stem cell and stem cell marker, and these findings play a very important role about exploitation the new therapy against synovial sarcoma.