Identification of synovial sarcoma initiating cells

我们证明细胞表面抗原A在形成滑膜肉瘤亚群的球体中高表达，并与干性基因的表达有关。A抗原阳性细胞具有较高的致瘤、自我更新和再流行活性，因此我们认为A抗原可以丰富滑膜肉瘤起始细胞。通过对42例滑膜肉瘤手术标本的免疫组织化学分析，发现A抗原表达与预后不良密切相关。此外，抗原A的特异性抑制物在体外也能抑制滑膜肉瘤细胞系的增殖。在本研究中，我们首先检测了滑膜肉瘤起始细胞和特异性标志物，这些发现对于开发滑膜肉瘤的新治疗方法具有非常重要的意义。

We demonstrated that the cell surface antigen A is highly expressed in the sphere forming subpopulation of synovial sarcoma and correlate with the expression of stemness genes. Antigen A positive cells possessed high tumorigenic, self-renewing, and re-popularizing activity; therefore we concluded that the Antigen A can enrich synovial sarcoma initiating cells. By immunohistochemical analysis of 42 synovial sarcoma surgical specimens, Antigen A expression was well correlated with poor prognosis. Moreover, the specific inhibitor of antigen A could suppress the proliferation of synovial sarcoma cell-lines in vitro.In this study, we first detect synovial sarcoma initiating cells and a specific marker, and these findings play a very important role about exploitation the new therapy against synovial sarcoma.