A novel urinary marker for detection of urothelial carcinoma The clinical significance and the biological role of 80KDa glycoprotein

一种检测尿路上皮癌的新型尿液标记物 80KDa 糖蛋白的临床意义和生物学作用

• 批准号: 23592341
• 负责人: KIMURA Taichi
• 金额: $ 3.24万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592341/
• 关键词: 腫瘍学; 尿路上皮癌; 尿中マーカー

Western blot analysis was performed using urine of the patients with bladder cancer (about 100 cases) by using the antibody reacts to the 80KDa glycoprotein (we detected previously). The result was revealed that these expression levels of 80KDa glycoprotein were higher in the advanced pathological grade cases statistifically significance.The real-time PCR revealed that mRNA level of 80KDa glycoprotein was higher in the order of normal urothelial cell line, low-grade urothelial cancer cell line and high-grade urothelial cancer cell lines.Treatment with histone deacetylase inhibitor and DNA methylation inhibitor restored LTF expression in utohelial cancer cell lines. Immunoreactivity of the 80KDa glycoprotein was undistinguished between cancer cells and normal urothelial cells in radical cystectomy specimens.

使用抗体对80KDA糖蛋白的反应，使用膀胱癌患者的尿液（约100例）进行了蛋白质印迹分析（我们先前检测到）。结果表明，在高级病理级别的病例中，这些80KDA糖蛋白的表达水平较高。恢复的LTF表达在Utohelial癌细胞系中。 80KDA糖蛋白的免疫反应性在自由基膀胱切除术标本中癌细胞和正常尿路上皮细胞之间没有区别。