n vivo imaging of bone and soft tissue tumor using fluorescent antibody

使用荧光抗体对骨和软组织肿瘤进行体内成像

• 批准号: 21791387
• 负责人: HAYASHI Katsuhiro
• 金额: $ 1.25万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791387/
• 关键词: 骨・軟部腫瘍学; 蛍光イメージング; 癌; 外科

We have investigated in vivo imaging of bone and soft tissue tumor using fluorescent antibody. Osteosarcoma cells were stained with fluorescent anti-ALP antibody. For distinction of normal tissue, red or near-infrared wavelength was suitable. Osteosarcoma cells were transplanted into mice and ICG was intravenously administered. Tumor was visualized by ICG. It can be used for tumor resection guide. Further investigation would be considered, such as improvement of observation devices.

我们研究了用荧光抗体对骨和软组织肿瘤的体内显像。用抗alp荧光抗体对骨肉瘤细胞进行染色。正常组织的鉴别，以红光或近红外波长为宜。将骨肉瘤细胞移植到小鼠体内，并静脉注射ICG。ICG显示肿瘤。可用于肿瘤切除指导。将考虑进一步的调查，例如改进观察设备。

项目成果

A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis

• DOI: 10.1111/j.1365-2184.2008.00574.x
• 发表时间: 2009-02-01
• 期刊: CELL PROLIFERATION
• 影响因子: 8.5
• 作者: Hayashi, K.;Yamauchi, K.;Hoffman, R. M.
• 通讯作者: Hoffman, R. M.

蛍光蛋白を使用した悪性腫瘍の転移リアルタイムイメージング

利用荧光蛋白对恶性肿瘤转移进行实时成像

• 发表时间: 2009
• 作者: 林克洋;土屋弘行;林克洋;林克洋;林克洋;林克洋;林克洋
• 通讯作者: 林克洋

Real-Time Imaging of Single Cancer-Cell Dynamics of Lung Metastasis

• DOI: 10.1002/jcb.22379
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF CELLULAR BIOCHEMISTRY
• 影响因子: 4
• 作者: Kimura, Hiroaki;Hayashi, Katsuhiro;Hoffman, Robert M.
• 通讯作者: Hoffman, Robert M.

Targeting of primary tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium

鼠伤寒沙门氏菌肿瘤选择性株靶向裸鼠原发肿瘤和高级别骨肉瘤肺转移

• 发表时间: 2010
• 作者: Hayashi K;et al
• 通讯作者: et al

Real Time Imaging of Cancer Cell Seeding to the Lung in Live Mouse

活体小鼠肺部癌细胞播种的实时成像

• 发表时间: 2009
• 作者: 林克洋;土屋弘行;林克洋
• 通讯作者: 林克洋