Regeneration of spinal cord injury using microRNA

在小鼠脊髓损伤后12小时和3天，用聚合酶链式反应检测到MicroRNA223的高水平表达。在脊髓损伤后12h，microRNA-223和炎性细胞因子在震中周围头部和尾部2 mm范围内也有明显的高水平表达。免疫组织化学显示60%的microRNA-223阳性细胞与Gr-1阳性的中性粒细胞融合。因此，中性粒细胞和炎性细胞因子的聚集发生在震中周围头部和尾部2 mm的范围内。提示在脊髓损伤的早期反应中，microRNA-223与中性粒细胞的表达有关。

MicroRNA223 shows high levels of expression at 12 hours and 3days after spinal cord injury using PCR in mice. And microRNA-223 and inflammatory cytokines also shows significantly high levels of expression within the range of 2mm of the head and caudal around the epicenter at 12 hours after spinal cord injury by PCR. Immunohistochemistry revealed 60% of the microRNA-223 positive cells merged with the Gr-1 positive neutrophils. Therefore, the accumulation of the neutrophils and inflammatory cytokine occurs within the range of the 2mm of head and caudal around the epicenter. And it was suggested that microRNA-223 is related to the expression of neutrophils in the early response of spinal cord injury.