Impact of anesthetic-induced preconditioning on mitochondrial ion channels

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We studied electrophysiological profiles of mitochondrial ion channels, which are known as important factors in anesthetic-induced preconditioning. Mytoplasts were obtained from isolated rat hearts by osmotic shock. Mitochondrial KATP channel were not detected in this study. We identified a mitochondrial ion channel with a conductance of 110pS in equimolar 150mM KCL solution. This channel current was removed when the 150mM KCL bath solution was replaced with 150mM K-glutamate. When 150mM TEA-Cl was used as bath solution, the current was not affected. In addition, The current was inhibited by DIDS, a chloride channel blocker. These results suggested that the mitochondrial ion channel was IMAC (inner membrane anion channel).