Gene-expression analysis of AR-negative prostate cancer cells

为了鉴定不依赖于PSA的前列腺癌特异性生物标志物，我们采用全基因组cDNA芯片分析了前列腺癌细胞系（PC-3、DU145、22Rv1和LNCaP）的基因表达谱，发现ar阴性的前列腺癌细胞系（PC-3和DU145）与ar阳性的前列腺癌细胞系（22Rv1和LNCaP）相比，存在数十个反式激活基因。其中，我们发现一个新的生物标志物KURO3在PC-3细胞系和临床高级别前列腺癌中是过表达基因。

To identify prostate cancer (PC) specific biomarkers, which are independent of PSA, we analyzed gene expression profiles of PC cell lines (PC-3, DU145, 22Rv1 and LNCaP) by genome-wide cDNA microarray and found dozens of trans-activated genes in AR-negative PC cell lines (PC-3 and DU145), compared with AR-positive PC cell lines (22Rv1 and LNCaP). Among them, we identified a new biomarker, KURO3, as an overexpressed gene in PC-3 cell line and clinical high-grade prostate cancers.