Regulation of trophoblast invasion by N-acetylglucosaminyltransferase in human placenta

N-乙酰氨基葡萄糖基转移酶V(GnT-V)在早孕胎盘绒毛外滋养层细胞(EVT)中有表达。我们利用EVT细胞株HTR-8/SVneo研究了GnT-V在EVT细胞中表达的调节因素。蜕膜组织培养上清液使GnT-V表达降低，证实转化生长因子-β是细胞因子中的因子。低氧(1%氧气)培养48h后，与常氧相比，GnT-V在mRNA和蛋白水平的表达均增强。由于GnT-V通过整合素a5b1的糖基化来调节滋养层细胞的侵袭，我们研究了与纤维连接蛋白孵育后对FAK磷酸化的影响。结果表明，与对照组细胞相比，GnT-V shRNA转染体中FAK的磷酸化水平降低。此外，我们还观察了正常胎盘和妊娠高血压综合征胎盘中GnT-V的表达差异，但免疫组织化学和免疫印迹显示GnT-V表达无差异。

N-acetylglucosaminyltransferase V (GnT-V) is expressed in extravillous trophoblast (EVT) of human placenta in the first-trimester. We investigated factors which regulate expression of GnT-V in EVT cells using EVT cell line, HTR-8/SVneo. The supernatant of decidual tissue culture made GnT-V expression reduced and we confirmed TGF-b was the factor in cytokines. Hypoxia (1% Oxygen) promoted GnT-V expression in mRNA and protein levels after incubation for 48 hours compared to normoxia. Because GnT-V regulates trophoblast invasion by glycosylation of integrina5b1, we investigated the affection on phosphorylation of FAK after incubation with fibronectin. The result was that phosphorylation of FAK was reduced in GnT-V shRNA transfectants copared to control cells. We also investigated the difference of GnT-V expression between normal placentas and pregnancy-induced hypertension's ones, but there was no difference in immunohistochemistry and Western blot.