The mechanism of carcinogenesis of choriocarcinoma with glycosyltransferases for hyperglycosylated hCG

高糖基化 hCG 糖基转移酶致癌绒毛膜癌的机制

• 批准号: 23592445
• 负责人: YAMAMOTO Eiko
• 金额: $ 3.33万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592445/
• 关键词: 絨毛癌; 糖転移酵素; GnT-IVa; C2GnT-1; C2GnT; 胞状奇胎; 細胞株樹立

The structures of sugar chains of hCG are quite different in trophoblastic neoplasia compared with normal pregnancy and hydatidiform mole, and it is because of N-acetylglucosaminyltransferase IVa (GnT-IVa)and core 2 beta-1, 6-N-acetylglucosaminyltransferase (C2GNT). The expression of GnT-IVa and C2GNT was strong in choriocarcinoma and invasive mole, but very weak in normal placenta and hydatidiform mole. The two glycosyltransferases involoved in promoting of invasion ability in choriocarcinoma. The result of immunoprecipitation suggested that Gnt-IVa promotes invasion ability via glycosylation of beta1 integrin. Luiferase assay suggested that cRel may regulate transcription of GnT-IVa via binding to the promoter of GnT-IVa.

滋养细胞肿瘤中hCG糖链结构与正常妊娠和葡萄胎相比有很大的不同，这是由于N-乙酰氨基葡萄糖转移酶IVa（GnT-IVa）和核心2 β-1，6-N-乙酰氨基葡萄糖转移酶（C2 GNT）的存在。GnT-IVa和C2 GNT在绒毛膜癌和侵蚀性葡萄胎中表达较强，在正常胎盘和葡萄胎中表达较弱。两种糖基转移酶参与绒毛膜癌侵袭能力的增强。免疫沉淀结果表明Gnt-IVa通过β 1整合素的糖基化促进侵袭能力。荧光素酶分析表明cRel可能通过与GnT-IVa启动子结合来调控GnT-IVa的转录。

项目成果

High expression of N-acetylglucosaminyltransferase IVa promotes invasion of choriocarcinoma.

• DOI: 10.1038/bjc.2012.496
• 发表时间: 2012-12-04
• 期刊: BRITISH JOURNAL OF CANCER
• 影响因子: 8.8
• 作者: Niimi, K.;Yamamoto, E.;Fujiwara, S.;Shinjo, K.;Kotani, T.;Umezu, T.;Kajiyama, H.;Shibata, K.;Ino, K.;Kikkawa, F.
• 通讯作者: Kikkawa, F.

N-acetylglucosam inyltransferase IVa regulates cell invasiveness of choriocarcinoma cells

N-乙酰葡萄糖酰基转移酶IVa调节绒毛膜癌细胞的细胞侵袭能力

• 发表时间: 2011
• 作者: K Niimi;E Yamamoto;K Shinjo;M Higashi;S Kondo and F Kikkawa
• 通讯作者: S Kondo and F Kikkawa

Role of N-acetylglucosaminyltransferase IVa in choriocarcinoma

N-乙酰氨基葡萄糖转移酶 IVa 在绒毛膜癌中的作用

• 发表时间: 2012
• 作者: Kaoru Niimi;Eiko Yamamoto;Kanako Shinjo;Sawako Fujiwara;Tomomi Kotani and Fumitaka Kikkawa
• 通讯作者: Tomomi Kotani and Fumitaka Kikkawa

An HLA-modified ovarian cancer cell line induced CTL responses specific to an epitope derived from claudin-1 presented by HLA-A*24:02 molecules

HLA 修饰的卵巢癌细胞系诱导针对 HLA-A*24:02 分子呈递的claudin-1 衍生表位特异的 CTL 反应

• DOI: 10.1016/j.humimm.2013.06.030
• 发表时间: 2013
• 期刊: Hum Immunol
• 影响因子: 2.7
• 作者: Kondo S;Demachi-Okamura A;Hirosawa T;Maki H;Fujita M;Uemura Y;Akatsuka Y;Yamamoto E;Shibata K;Ino K;Kikkawa F;Kuzushima K.
• 通讯作者: Kuzushima K.

The Loss of Endoglin Promotes the Invasion of Extravillous Trophoblasts

• DOI: 10.1210/en.2011-1088
• 发表时间: 2011-11-01
• 期刊: ENDOCRINOLOGY
• 影响因子: 4.8
• 作者: Mano, Yukio;Kotani, Tomomi;Kikkawa, Fumitaka
• 通讯作者: Kikkawa, Fumitaka