Elucidation of a gene-specific regulatory mechanism with progesterone in the human endometrial stromal cells

ChIP法显示TNFα可增加NF-κB在COX-2和Mn-SOD启动子的结合，孕酮仅抑制NF-κB在考克斯-2启动子的结合。Mn-SOD启动子的NF-κB反应元件的组蛋白乙酰化水平低于考克斯-2启动子。孕酮的基因特异性作用可能是由于考克斯-2启动子和Mn-SOD启动子中NF-κB反应元件的染色质结构不同。

ChIP assay revealed that TNFα increased NF-κB binding at both the COX-2 promoter and the Mn-SOD promoter, and that progesterone only inhibited the NF-κB binding at the COX-2 promoter. The histone acetylation level of the NF-κB response element of the Mn-SOD promoter was lower than that of the COX-2 promoter. The gene-specific action of progesterone may be due to the difference in chromatin structure at the NF-κB response elements in the COX-2 promoter and Mn-SOD promoter.