Analysis of pneumococcal surface protein and development of pneumococcal vaccine inhibiting colonization in the upper respiratory tract

肺炎球菌表面蛋白分析及抑制上呼吸道定植的肺炎球菌疫苗的研制

• 批准号: 21791639
• 负责人: SAKAI Akihiro
• 金额: $ 2.58万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791639/
• 关键词: 耳科学; 肺炎球菌; PspA; ワクチン; 急性中耳炎; 急性副鼻腔炎; 耐性化

We analyzed the pneumococcal surface proteins of pneumococcal strains from children with otitis media by PCR assay. Recombinant pneumococcal surface proteins were made by TA cloning method. Pneumococcal colonization in the nasopharynx was prevented by intranasal immunization with recombinant pneumococcal surface protein in the mouse model. Cross-protective-reactivity between pneumococcal surface protein families were shown in the systemic infection model. Intranasal immunization with pneumococcal surface protein is important about the development of novel vaccines in the near future.

我们用聚合酶链反应（PCR）分析了儿童中耳炎肺炎球菌的表面蛋白。用TA克隆法制备重组肺炎球菌表面蛋白。在小鼠模型中，通过用重组肺炎球菌表面蛋白鼻内免疫来预防肺炎球菌在鼻咽中的定植。在系统性感染模型中，肺炎球菌表面蛋白家族之间存在交叉保护反应。肺炎球菌表面蛋白的鼻内免疫对于未来新型疫苗的开发具有重要意义。

项目成果

小児鼻咽腔における肺炎球菌血清型の定量的検討

儿童鼻咽部肺炎球菌血清型的定量研究

• 发表时间: 2009
• 作者: Tadao Okayasu;Tadashi Nishimura;Akinori Yamashita;Seiji Nakagawa;Yoshiki Nagatani;Shuichi Yanai;Yuka Uratani;Hiroshi Hsoi;Ogasawara N;小上真史
• 通讯作者: 小上真史