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Research for associations of tension-dependent aqueous outflow regulation by trabecular meshwork with glaucoma pathology.

小梁网张力依赖性房水流出调节与青光眼病理学关联的研究。

基本信息

批准号：
21791693
负责人：
INOUE Toshihiro
金额：
$ 2.25万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2010
项目状态：
已结题

项目摘要

To identify factors associated with the tension-dependency, we developed a pressure model by using three-dimension culture of trabecular meshwork cells. In this model, collagen, medium, and cells were mixed and then were stabilized by changing the pH. After letting cells migrate and proliferate in the model, the model was weighed, and cells in the model were observed by immunostaining. F-actin was found to be decreased by observation with the confocal microscopy. Considering ROCK inhibitors which decrease F-actin could lower intraocular pressure in human, we assumed that this response contributed to the homeostasis of intraocular pressure by using the same mechanism with ROCK inhibitors. It was difficult, however, to approach proteins in cells in the three-dimension culture. We therefore assessed the reaction of trabecular cells against oxidative stress, because we speculated that the intracellular reaction against pressure stress had similar aspects with that against oxidative stress. After exposure to oxidative stress, the Akt-PI3K signaling and the p38 signaling in the trabecular meshwork cells were revealed to be activated. Moreover, oxidative stress-induced cell death was enhanced by inhibiting these signalings. These novel findings provided us a valuable clue to understanding the mechanisms that regulate aqueous humor outflow.

为了确定与张力依赖相关的因素，我们利用小梁网细胞的三维培养建立了压力模型。本模型将胶原、培养基、细胞混合，改变ph稳定，待细胞在模型中迁移增殖后称重，免疫染色观察模型细胞。共聚焦显微镜观察发现f -肌动蛋白减少。考虑到降低f -肌动蛋白的ROCK抑制剂可以降低人眼压，我们假设这种反应与ROCK抑制剂的机制相同，有助于眼压的内稳态。然而，在三维培养中接近细胞中的蛋白质是很困难的。因此，我们评估了小梁细胞对氧化应激的反应，因为我们推测细胞内对压力应激的反应与对氧化应激的反应有相似的方面。氧化应激后，小梁网细胞中的Akt-PI3K信号和p38信号被激活。此外，氧化应激诱导的细胞死亡通过抑制这些信号而增强。这些新发现为我们理解房水流出调节机制提供了有价值的线索。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

RGS2-deficient mice exhibit decreased intraocular pressure and increased retinal ganglion cell survival.

RGS2缺陷小鼠表现出眼内压降低和视网膜神经节细胞存活增加。

DOI：
发表时间：
2009
期刊：
Molecular vision
影响因子：
2.2
作者：
Inoue-Mochita,Miyuki;Inoue,Toshihiro;Epstein,DavidL;Blumer,KendallJ;Rao,PonugotiV
通讯作者：
Rao,PonugotiV

Trabeculectomy with mitomycin C for open-angle glaucoma in phakic eyes vs pseudophakic eyes after phacoemulsification.

使用丝裂霉素 C 进行小梁切除术治疗有晶状体眼与白内障超声乳化术后人工晶状体眼的开角型青光眼。

DOI：
发表时间：
2011
期刊：
Archives of Ophthalmology 129
影响因子：
0
作者：
Takihara Y;Inatani M;Seto T;Iwao K;Iwao M;Inoue T;Kasaoka N;Murakami A;Futa R;Tanihara H.
通讯作者：
Tanihara H.

Risk Factors for Secondary Glaucoma After Vitrectomy for Proliferative Diabetic Retinopathy.

增殖性糖尿病视网膜病变玻璃体切除术后继发性青光眼的危险因素。