Comprehensive investigation of tension-sensitive factors that related to changes in cytoskeleton and cell adhesion in trabecular meshwork cells

小梁网细胞细胞骨架和细胞粘附变化相关张力敏感因子的综合研究

• 批准号: 23791994
• 负责人: INOUE Toshihiro
• 金额: $ 2.66万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791994/
• 关键词: 緑内障; 線維柱帯; 酸化ストレス; 圧感受性; 細胞骨格; 房水; 線維柱体; シュレム管内皮

We made a device to add hydrostatic pressure on cultured cells, resulting in changes in cell morphology even in the control condition, and the data were not analyzable. After treatment of trabecular meshwork cells with Y-27632, 5 genes that were related to antioxidant functions were up-regulated. Of those, the up-regulation of catalase was confirmed by realtime RT-PCR. Pretreatment with Y-27632 increased the signal of WST-8 assay and decreased the signal of DCFDA test reagent under stimulation with hydrogen peroxide. Collectively, it was indicated that ROCK inhibitor induced intracellular expression of antioxidants in the trabecular meshwork cells, and thereby suppressed oxidative stress-induced cell death.

我们制作了一个对培养细胞施加静水压力的装置，即使在控制条件下，细胞形态也会发生变化，数据无法分析。用Y-27632处理小梁网细胞后，5个与抗氧化功能相关的基因上调。其中过氧化氢酶的上调通过实时RT-PCR证实。Y-27632预处理后，过氧化氢刺激下WST-8检测信号升高，DCFDA检测试剂信号降低。综上所述，ROCK抑制剂可诱导小梁网细胞内抗氧化剂的表达，从而抑制氧化应激诱导的细胞死亡。

项目成果

Evaluation of filtering blebs with transconjunctival oozing by anterior segment optical coherence tomography

眼前段光学相干断层扫描评估经结膜渗出的滤过泡

• 发表时间: 2013
• 作者: Nakashima K;Inoue T;Fukushima A;Hirakawa S;Kawaji T;Tanihara H
• 通讯作者: Tanihara H

Filtration Bleb-associated Tear Film Sign Demonstrated by Three Dimensional Anterior Segment Optical Coherence Tomography (3D AS-OCT).

三维眼前段光学相干断层扫描 (3D AS-OCT) 显示滤过泡相关泪膜征象。

• 发表时间: 2012
• 作者: Tsuboi N;Inoue T;Fujimoto T;Inoue-Mochita M;Kasaoka N;Kawai M;Shobayashi K;Yoshida A;Tanihara H;Kojima S;Kawai M;Tanihara H;Fujimoto T;Tsuboi N;Takihara Y;Kojima S
• 通讯作者: Kojima S

Study on prognostic factor in trabeculectomy with mitomycin C having history of previous glaucoma surgery

有青光眼手术史的丝裂霉素C小梁切除术的预后因素研究

• DOI: 10.1007/s10384-013-0257-5
• 发表时间: 2013
• 期刊: Japanese Journal of Ophthalmology
• 影响因子: 2.4
• 作者: Awai-Kasaoka N;Inoue T;Inatani M;Takihara Y;Ogata-Iwao M;Tanihara H
• 通讯作者: Tanihara H

線維柱帯細胞においてROCK阻害薬が酸化ストレスに与える影響

ROCK抑制剂对小梁网细胞氧化应激的影响

• 发表时间: 2013
• 作者: Fujimoto T;Inoue T;Inoue-Mochita M;Kasaoka N;Shobayashi K;Tanihara H;笠岡奈々子,井上俊洋,亀田隆範,藤本智和,井上みゆき,谷原秀信
• 通讯作者: 笠岡奈々子,井上俊洋,亀田隆範,藤本智和,井上みゆき,谷原秀信

Monocyte chemotactic protein-1 level in the aqueous humour as a prognostic factor for the outcome of trabeculectomy

• DOI: 10.1111/ceo.12204
• 发表时间: 2014-05-01
• 期刊: CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
• 影响因子: 4
• 作者: Inoue, Toshihiro;Kawaji, Takahiro;Tanihara, Hidenobu
• 通讯作者: Tanihara, Hidenobu