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Application of Laser to Endodontic Micro-Surgery

激光在牙髓显微手术中的应用

基本信息

批准号：
21791837
负责人：
SAEGUSA Hidetoshi
金额：
$ 2.5万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

1 : Initial fibroblast cell attachment to Erbium : YAG laser-irradiated dentinThe purpose of this study was to evaluate the biocompatibilities of resected dentin surfaces after treatment with acid, low-level Er : YAG laser irradiation, or laser irradiation plus antibiotics. Dentin disks obtained from single-rooted human teeth were divided into the following groups :(1) Laser group, treated with Er : YAG laser irradiation(30mJ/pulse, 10pps, 60s);(2) L-MTAD group, treated with laser irradiation as in the laser group plus a mixture of doxycycline, tetracycline isomer and citric acid ;(3) RC-Prep group, treated with EDTA gel or cream(RC-Prep); and(4) Control group, left untreated. After each treatment, the dentin disks were incubated with NIH/3T3 fibroblasts cultured to subconfluency in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and antibiotics. The number of attached cells among the groups was statistically analyzed at the 5% significance level. The dentin … More surface morphologies was observed under scanning electron microscopy(SEM). Cell attachments were evaluated at three representative time points : 4, 8, and 16h following cell culture treatment by counting assays, histological observation, and SEM. The number of attached cells was significantly higher(p<0. 05) in the Laser group than in the RC-Prep and Control groups at 16h. Dendritic cell extension of the fibroblasts was only observed in the Laser group at 8h by SEM. In the histological analyses, significantly more attached cells were found on the dentin surfaces treated with laser irradiation.2 : Effects of dentin surface modifications treated with Er : YAG and Nd : YAG laser irradiation on fibroblast cell adhesionThis in vitro study evaluated fibroblast adhesion to dentin surfaces treated with Er : YAG and Nd : YAG laser irradiation by analyzing the morphology and roughness of dentin surfaces and compared the results with those of the conventional treatment with burs. Thirty-five freshly extracted single-rooted human teeth with fully formed apices were used in this study. The root apex of each tooth was removed and dentin disks were prepared using a low-speed saw. Dentin disks were divided into three groups. Group A was left untreated, Group B was treated with Er : YAG laser irradiation(60mJ/pulse, 10 pps, 60s), and Group C was treated with Nd : YAG laser irradiation(60mJ/pulse, 10pps, 60s). After laser irradiation, the dentin disks were incubated with NIH/3T3 fibroblasts cultured in Dulbecco's modified Eagle's medium. A morphological analysis of the dentin surface was observed under SEM, and surface roughness was measured using a confocal laser scanning microscopy. Cell adhesion was evaluated at 12and 24h of incubation periods using SEM analysis and WST assay. The statistical analysis was undertaken using ANOVA at a 5% level of significance(p<0. 05). Morphological analysis and roughness measurement showed that all dentin surfaces treated with Er : YAG laser irradiation(Group B) were rougher than those in Groups A and C. In the Er : YAG-irradiated group, attached cells were flattened and attached to the dentin by expanded filopodia after 12h of culture. Group B(Er : YAG) exhibited the greatest number of attached cells among all groups after 12 and 24h. Less

图1：初始成纤维细胞附着于铒：YAG激光照射的牙本质本研究的目的是评估用酸、低水平Er：YAG激光照射或激光照射加抗生素处理后切除的牙本质表面的生物相容性。将人单根牙获得的牙本质盘分为以下几组：（1）激光组，采用Er：YAG激光照射（30mJ/脉冲，10pps，60s）；（2）L-MTAD组，采用与激光组相同的激光照射加多西环素、四环素异构体和柠檬酸的混合物；（3）RC-Prep 组，用EDTA凝胶或霜(RC-Prep)治疗； (4)对照组，不予治疗。每次处理后，将牙本质盘与 NIH/3T3 成纤维细胞一起孵育，在补充有 10% 胎牛血清和抗生素的 Dulbecco 改良 Eagle 培养基中培养至亚汇合。以5%显着性水平对各组间贴壁细胞数进行统计分析。在扫描电子显微镜（SEM）下观察牙本质表面形态。通过计数测定、组织学观察和 SEM 在细胞培养处理后的 4、8 和 16 小时三个代表性时间点评估细胞附着。 16小时时，激光组贴壁细胞数量显着高于RC-Prep组和对照组（p<0. 05）。仅在激光组中 8 小时时通过 SEM 观察到成纤维细胞的树突状细胞延伸。在组织学分析中，在经激光照射处理的牙本质表面上发现显着更多的附着细胞。 2：经 Er : YAG 和 Nd : YAG 激光照射处理的牙本质表面修饰对成纤维细胞粘附的影响本体外研究评估了经 Er : YAG 和 Nd : YAG 激光照射处理的牙本质表面成纤维细胞的粘附，分析牙本质表面的形态和粗糙度，并将结果与传统车针治疗的结果进行比较。本研究使用了 35 颗新拔出的具有完全形成的牙尖的单根人类牙齿。去除每颗牙齿的根尖并使用低速锯制备牙本质盘。牙本质盘分为三组。 A组不予治疗，B组采用Er:YAG激光照射(60mJ/脉冲，10pps，60s)，C组采用Nd:YAG激光照射(60mJ/脉冲，10pps，60s)。激光照射后，将牙本质盘与在 Dulbecco 改良 Eagle 培养基中培养的 NIH/3T3 成纤维细胞一起孵育。在扫描电镜下观察牙本质表面的形态分析，并使用共焦激光扫描显微镜测量表面粗糙度。使用 SEM 分析和 WST 测定在 12 小时和 24 小时孵育期间评估细胞粘附。使用 ANOVA 以 5% 显着性水平进行统计分析 (p<0. 05)。形态学分析和粗糙度测量表明，Er:YAG激光照射组（B组）的牙本质表面均比A组和C组粗糙。Er:YAG激光照射组中，培养12h后，贴壁细胞变平，通过扩张的丝状伪足附着在牙本质上。 12 小时和 24 小时后，B 组（Er：YAG）在所有组中表现出最多数量的贴壁细胞。较少的