Inhibition of Pin1 activity suppresses tumor growth in oral cancer.

(1)建立过表达Pin1的口腔癌细胞系。该细胞系的生长速度上调。(2)在该细胞系中，Cyclin D1的表达水平也上调。(3)我们在口腔癌、结肠癌、卵巢癌和肝癌细胞系中检测了Pin1蛋白水平。Pin1在HCC中的表达水平低于其他肿瘤。(4)所有细胞系均未发现突变。(5)使用Pin1抑制剂处理48hr时，S期细胞数量增加。然而，没有特定的细胞周期阻滞。

(1) We established oral cancer cell line with Pin1 overexpression. The growth rate of this cell line was up-regulated. (2) In this cell line, Expression level of Cyclin D1 was also up-regulated. (3) We detected Pin1 protein levels with some kinds of cancer cell line including oral cancer, colon cancer, ovarian cancer and hepatocellular carcinoma. The expression level of Pin1 in HCC was lower than that of other cance. (4) No mutation was found in all of those cell line. (5) Treatment with Pin1 inhibitor increased the population of cells in S phase at 48hr. However, there was not a specific cell cycle arrest.