Basic resarch about application of targeted therapy by anaysis of cancer-associated gene in oral squamous cell carcinoma

我们用ARRAY-CGH检测了21个口腔鳞状细胞癌细胞系中拷贝数的变化，在TOSCA 55细胞系中检测到6q22左右的高水平扩增，FISH分析发现约3.5Mb的扩增区域。根据基因组数据库的信息，在该区域定位了8个基因，然后测定了21个口腔鳞状细胞癌细胞系中8个基因的mRNA表达水平，其中3个基因在大多数口腔鳞癌细胞系中表达，表明这些基因是6q22扩增区域的靶点。

We assessed copy-number alterations among the 21 OSCC cell lines by array-CGH .High-level amplifications were detected in Tosca 55 cell lines around 6q22.By FISH analysys,We recognized around 3.5 Mb amplification region. In this region,eight genes located according to the information archived by genome detabases.Next we determined the mRNA expression levels of eight genes in 21 OSCC cell lines .Among eight genes,three genes were expressed in most of the OSCC cell lines,suggesting that these genes are the target for 6q22 amplification region.