Basic resarch about application of targeted therapy by anaysis of cancer-associated gene in oral squamous cell carcinoma

癌相关基因分析在口腔鳞癌靶向治疗中应用的基础研究

• 批准号: 21791977
• 负责人: NAKAMURA Erina
• 金额: $ 1.41万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791977/
• 关键词: 口腔外科学一般; ゲノム構造異常; 口腔癌; アレイ解析

We assessed copy-number alterations among the 21 OSCC cell lines by array-CGH .High-level amplifications were detected in Tosca 55 cell lines around 6q22.By FISH analysys,We recognized around 3.5 Mb amplification region. In this region,eight genes located according to the information archived by genome detabases.Next we determined the mRNA expression levels of eight genes in 21 OSCC cell lines .Among eight genes,three genes were expressed in most of the OSCC cell lines,suggesting that these genes are the target for 6q22 amplification region.

利用阵列CGH技术检测了21个口腔鳞癌细胞系的DNA拷贝数变化，发现Tosca 55细胞系在6q22附近有高水平的扩增，FISH分析发现扩增区域在3.5Mb左右。在此区域内，我们根据基因组数据库的信息定位了8个基因，并检测了8个基因在21个口腔鳞癌细胞系中的mRNA表达水平，其中3个基因在绝大多数口腔鳞癌细胞系中均有表达，表明这些基因是6q22扩增区域的靶基因。

项目成果

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Frequent silencing of a putative tumor suppressor gene melatonin receptor 1 A (MTNR1A) in oral squamous-cell carcinoma

• DOI: 10.1111/j.1349-7006.2008.00838.x
• 发表时间: 2008-07-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Nakamura, Erina;Kozaki, Ken-ichi;Imoto, Issei
• 通讯作者: Imoto, Issei