Basic resarch about application of targeted therapy by anaysis of cancer-associated gene in oral squamous cell carcinoma

我们通过阵列- cgh评估了21种OSCC细胞系的拷贝数变化。在Tosca 55细胞系6q22附近检测到高水平扩增。通过FISH分析，我们识别出约3.5 Mb的扩增区。在这个区域，根据基因组数据库存档的信息定位了8个基因。接下来，我们测定了8个基因在21个OSCC细胞系中的mRNA表达水平。在8个基因中，有3个基因在大多数OSCC细胞系中表达，提示这些基因是6q22扩增区的靶基因。

We assessed copy-number alterations among the 21 OSCC cell lines by array-CGH .High-level amplifications were detected in Tosca 55 cell lines around 6q22.By FISH analysys,We recognized around 3.5 Mb amplification region. In this region,eight genes located according to the information archived by genome detabases.Next we determined the mRNA expression levels of eight genes in 21 OSCC cell lines .Among eight genes,three genes were expressed in most of the OSCC cell lines,suggesting that these genes are the target for 6q22 amplification region.