Cooperative interactions of a subgroup of the R2R3-MYB transcription factors in controlling phenylpropanoid accumulation

与动物不同，植物含有MYB蛋白亚家族，其特征是r2r3型MYB dna结合域。拟南拟126个R2R3-MYB因子包含一个明确的MYB结构域，具有较高的保守性。该基因家族的成员控制着几个植物特有的过程，包括在逆境下苯丙素的积累。然而，约80%的r2r3型MYB因子的功能信息缺失。该项目旨在确定R2R3-MYB转录因子子集在控制苯丙类积累中的合作相互作用。我们将确定r2r3型MYB因子家族成员的积累和定位模式。在植物原生质体中，通过系统的共转染实验来研究合作相互作用的功能。此外，在植物中的相互作用将通过使用表位标记的MYB因子变体来研究。标记的MYB蛋白，当它们被证明可以补充相应的敲除表型时，将被用于识别预期的和潜在的新的相互作用蛋白。

In contrast to animals, plants contain a MYB protein subfamily which is characterised by the R2R3-type MYB DNA-binding domain. The 126 R2R3-MYB factors of A. thaliana contain a clearly defined MYB domain with a high level of conservation. Members of this gene family control several plant-specific processes including the accumulation of phenlypropanoids in response to stress. However, for about 80 % of the R2R3-type MYB factors functional information is missing. The proposed project aims at defining cooperative interactions of a subset of the R2R3-MYB transcription factors in controlling phenylpropanoid accumulation. We will determine accumulation and localisation patterns for members of the R2R3-type MYB factor family. Cooperative interactions will be functionally investigated by systematic co-transfection assays in plant protoplasts. In addition, in planta interactions will by studied by using epitope-tagged MYB factor variants. The tagged MYB proteins will, when they have been shown to complement the respective knock-out phenotype, be used to identify expected and potentially new interacting proteins.