Molecular mechanism of inhibition of insect odor-gated ion channels

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Insect olfactory receptors compose heteromeric ion channels activated by odorants. Receptor screening experiments revealed the genes encoding ionotropic receptors. I expressed the receptors in heterologous cells, and analyzed their activity. I found some compounds competed with a ligand at their binding site on receptors. The receptor screening of silk moth genome showed a sugar-regulated ion channel, in which structurally related sugar inhibited its binding site. Finally, mutagenesis experiment of olfactory receptor complex identified the amino acid residue contributing the function of channel pore formation.