Relationship between Biophysical Properties and Microstructure of Actin Stress Fibers

应激纤维在调节细胞内稳态中起关键作用。本文研究了它们的生物物理性质和微观结构。特别是，我们开发了一种方法来分离在结构（通过免疫印迹和电子显微镜证实）和功能（通过收缩性试验证实）方面完好无损的应力纤维。然后，我们提出了一个分子机制，可能是细胞缩短导致应力纤维断裂的基础。

Stress fibers play a key role in regulation of cell homeostasis. Here we investigated their biophysical properties and microstructure. Particularly, we developed a method to isolate stress fibers that are intact with regard to structure (confirmed by western blotting and electron microscopy)and function(confirmed by a contractility assay). We then submitted a molecular mechanism that may underlie the cell shortening-induced disassembly of stress fibers.