Relationship between Biophysical Properties and Microstructure of Actin Stress Fibers

应激纤维在细胞内稳态的调节中起着关键作用。在这里，我们研究了它们的生物物理性质和微观结构。特别是，我们开发了一种分离应力纤维的方法，这些纤维在结构(经Western blotting和电子显微镜证实)和功能(经收缩试验证实)方面是完整的。然后，我们提出了一个分子机制，可能是细胞缩短导致应力纤维解体的基础。

Stress fibers play a key role in regulation of cell homeostasis. Here we investigated their biophysical properties and microstructure. Particularly, we developed a method to isolate stress fibers that are intact with regard to structure (confirmed by western blotting and electron microscopy)and function(confirmed by a contractility assay). We then submitted a molecular mechanism that may underlie the cell shortening-induced disassembly of stress fibers.