CHEMOTAXIS OF HUMAN PERIODONTIUM DERIVED CELLS.

我们已经表明，条件培养基（HPLF-CM）增强附着和扩展的人牙周膜成纤维细胞（HPLF）。本研究旨在探讨人牙周组织来源的细胞（HPLF、HGF、HABC、HGE）和人外周血中性粒细胞（PMN）的趋化性、粘附性和铺展性。我们发现，HPLF-CM不仅刺激趋化性迁移，而且还刺激所有使用的人牙周组织来源的细胞的附着和铺展。HPLF的趋化性迁移受HPLF-CM浓度范围（35 μ g/ml-70 ng/ml）的刺激。通过PO-60 k柱分级分离，在分子量约为70 kDa的HPLF-CM中，HGF也显示出高的趋化活性。HPLF-CM在8.7 μ g-70 ng/ml时对HABC的趋化活性有促进作用，而在35 μ g/ml时则无此作用，但350、175 ng/ml的HPLF-CM与10 μ M的FMLP相比，对PMN的迁移无促进<-7>作用。

We have shown that the conditioned medium (HPLF-CM) enhanced attachment and spreading of human periodontal ligament fibroblast (HPLF). The present study was attempted to evaluate the chemotaxis, attachment and spreading of human periodontal tissue derived cells (HPLF,HGF,HABC,HGE) and human peripheral blood neutrophill (PMN). We found that HPLF-CM not stimulated only the chemotactic migration but also the attachment and spreading of all of used human periodontal tissue derived cells. HPLF was stimulated the chemotactic migration a wide range of HPLF-CM concentration (35 mug/ml-70 ng/ml). HGF was also indicated high chemotactic activity at a molecular weight of approximately 70 kDa of HPLF-CM as determined by fractioned in a PO-60k column. HABC was stimulated chemotactic activity with 8.7 mug-70 ng/ml HPLF-CM,but not with 35 mug/ml HPLF-CM.However, 350,175 ng/ml HPLF-CM were not enhanced the migration of PMN compared with 10^<-7> M FMLP.Hence we conclude that HPLF-CM contains the cell attachment, spreading and chemotactic factors, which may contribute to the development and regeneration of connective tissue attachment in vivo.