CHEMOTAXIS OF HUMAN PERIODONTIUM DERIVED CELLS.

人牙周组织衍生细胞的趋化性。

• 批准号: 05671608
• 负责人: DEGUCHI Shinji
• 金额: $ 1.41万
• 依托单位: KANAGAWA DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671608/
• 关键词: HUMAN PERIODONTIUM DERIVED CELLS; CHEMOTAXIS; CONDITIONED MEDIUM; CELL ATTACHMENT AND SPREADING; 歯周組織由来細胞; 歯根膜由来線維芽細胞; Conditioned mediom

We have shown that the conditioned medium (HPLF-CM) enhanced attachment and spreading of human periodontal ligament fibroblast (HPLF). The present study was attempted to evaluate the chemotaxis, attachment and spreading of human periodontal tissue derived cells (HPLF,HGF,HABC,HGE) and human peripheral blood neutrophill (PMN). We found that HPLF-CM not stimulated only the chemotactic migration but also the attachment and spreading of all of used human periodontal tissue derived cells. HPLF was stimulated the chemotactic migration a wide range of HPLF-CM concentration (35 mug/ml-70 ng/ml). HGF was also indicated high chemotactic activity at a molecular weight of approximately 70 kDa of HPLF-CM as determined by fractioned in a PO-60k column. HABC was stimulated chemotactic activity with 8.7 mug-70 ng/ml HPLF-CM,but not with 35 mug/ml HPLF-CM.However, 350,175 ng/ml HPLF-CM were not enhanced the migration of PMN compared with 10^<-7> M FMLP.Hence we conclude that HPLF-CM contains the cell attachment, spreading and chemotactic factors, which may contribute to the development and regeneration of connective tissue attachment in vivo.

我们已经表明，条件培养基（HPLF-CM）增强附着和扩展的人牙周膜成纤维细胞（HPLF）。本研究旨在探讨人牙周组织来源的细胞（HPLF、HGF、HABC、HGE）和人外周血中性粒细胞（PMN）的趋化性、粘附性和铺展性。我们发现，HPLF-CM不仅刺激趋化性迁移，而且还刺激所有使用的人牙周组织来源的细胞的附着和铺展。HPLF的趋化性迁移受HPLF-CM浓度范围（35 μ g/ml-70 ng/ml）的刺激。通过PO-60 k柱分级分离，在分子量约为70 kDa的HPLF-CM中，HGF也显示出高的趋化活性。HPLF-CM在8.7 μ g-70 ng/ml时对HABC的趋化活性有促进作用，而在35 μ g/ml时则无此作用，但350、175 ng/ml的HPLF-CM与10 μ M的FMLP相比，对PMN的迁移无促进<-7>作用。

项目成果

TAKAFUMI SAKAMOTO: "PARTIAL PURIFICATION OF CELL ATTACHMENT AND SPREADING FACTORS DERIVED FROM THE CONDITIONED MEDIUM OF HUMAN PERIODONTAL LIGAMENT FIBROBLAST.BULL OF KANAGAWA DENT.COL." 21 (1). 25-34 (1993)

Takafumi Sakamoto：“从人类牙周韧带成纤维细胞的条件培养基中提取的细胞附着和扩散因子的部分纯化。神奈川牙科大学的牛。”

出口眞二: "ヒト歯根膜由来線維芽細胞の化学走化性について" 日本歯周病学会会誌. 36. 563-570 (1994)

Shinji Deguchi：“人牙周膜来源的成纤维细胞的趋化性”日本牙周病学会杂志 36. 563-570 (1994)。

TETSUO MAKINO: "EFFECT OF L-ASCORBIC ACID,L-ASCORBIC ACID-2-PHOSPHATE AND GROWTH FACTORS ON THE CELLULAR RESPONSES OF FIBROBLASTS DERIVED FROM HUMAN DECIDUOUS AND PERMANENT PERIODONTAL LIGAMENTS.THE JOURNAL OF THE KANAGAWA ODONTOLOGICAL SOCIETY" 28 (3). 2

TETSUO MAKINO：“L-抗坏血酸、L-抗坏血酸-2-磷酸盐和生长因子对人类乳牙和永久牙周韧带来源的成纤维细胞反应的影响。神奈川牙科学会杂志”28 (3 ）。

川村太助: "コラーゲン・ゲル内培養におけるヒト歯根膜由来線維芽細胞の細胞接着・伸展因子の影響について" 神奈川歯学. 27. 543-558 (1993)

Taisuke Kawamura：“胶原凝胶中培养的人牙周膜来源的成纤维细胞的细胞粘附和扩散因子的影响”神奈川牙科科学 27. 543-558 (1993)。

RENTARO YOSHINO: "CELLULAR RESPONSES OF HUMAN PERIODONTAL DERIVED CELLS BY MECHANICAL STRESS.THE JOURNAL OF THE KANAGAWA ODONTOLOGICAL SOCIETY" 28 (2). 166-184 (1993)

吉野伦太郎：“机械应力对人类牙周衍生细胞的细胞反应。神奈川牙科学会杂志”28（2）。