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CHEMOTAXIS OF HUMAN PERIODONTAL DERIVED CELLS

人牙周来源细胞的趋化性

基本信息

批准号：
07672095
负责人：
DEGUCHI Shinji
金额：
$ 1.41万
依托单位：
KANAGAWA DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672095/
关键词：
HUMAN PERIODONTIUM DERIVED CELLS CHEMOTAXIS CONDITION MEDIUM CONNECTIVE TISSUE ATTACHMENT

项目摘要

At the completion of flap reflection, the soft tissues are placed in contact with the root surface. Epithelial and connective tissue reattachment is complete at 3 weeks. Freely mobile cells of the gingival lamina propria include fibroblast, mast cells, and a small number of mononuclear and polymorphonuclear leukocytes. The most numerous cell type is the fibroblast. Human gingival fibroblasts (HGFs) produce connective tissue substances including collagen, , proteoglycans, and elastin, and essentially maintain the integrity of gingival lamina propria. The present investigation was conducted evaluate the effect of human gingival fibroblasts conditioned medium (HGF-CM) to chemotaxis of human periodontal ligament fibroblasts (HPLFs), HGF and human alveolar bone cells (HABCs), and human gingival epithelial cells (HGEs).To determine whether HGF-CM was chemotactic, and/or chemokinetic for fibroblast, a checcurboard analysis of the Zigmond-Hirsch type was performed wherein different concentrations of HGF-CM were added to upper (cell) and lower (test) compartment of modified blind-well Boyden-type chamber, and migration of fibroblasts was measured. This analysis suggests that HGF-CM induced chemotactic migration of HGF and HPLF,because the cells migration increased in the presence of positive gradient, i.e.a higher of 175ng/ml concentration of the peptide in the lower compartment than in the upper compartment. HGF-CM consistently increased migration of HABC and HGE at concentration higher of 50% HGF-CM,similar result of HPLF-CM (100%) which was positive control. The data suggested that HGF-CM may contribute to the development of periodontal regeneration.

在完成皮瓣反射时，将软组织放置成与牙根表面接触。3周时上皮和结缔组织完全重新附着。牙龈固有层的自由移动的细胞包括成纤维细胞、肥大细胞和少量单核和多形核白细胞。数量最多的细胞类型是成纤维细胞。人牙龈成纤维细胞（HGF）产生结缔组织物质，包括胶原、蛋白多糖和弹性蛋白，并基本上维持牙龈固有层的完整性。本研究观察了人牙龈成纤维细胞条件培养液（HGF-CM）对人牙周膜成纤维细胞（HPLFs）、人牙槽骨细胞（HABCs）和人牙龈上皮细胞（HGEs）趋化性的影响，以确定HGF-CM对成纤维细胞是否具有趋化性和/或趋化动力学作用。进行Zigmond-Hirsch型的棋盘分析，其中将不同浓度的HGF-CM加入到改进的盲孔Boyden型室的上部（细胞）和下部（测试）隔室中，并测量成纤维细胞的迁移。这一分析表明，HGF-CM诱导的HGF和HPLF的趋化迁移，因为细胞迁移增加的存在下，正梯度，即175 ng/ml浓度的肽在较低的隔室比在较高的隔室。在高于50% HGF-CM的浓度下，HGF-CM一致地增加HABC和HGE的迁移，与作为阳性对照的HPLF-CM（100%）的结果相似。提示HGF-CM可能有助于牙周组织再生。