CHEMOTAXIS OF HUMAN PERIODONTAL DERIVED CELLS

在完成皮瓣反射时，将软组织放置成与牙根表面接触。3周时上皮和结缔组织完全重新附着。牙龈固有层的自由移动的细胞包括成纤维细胞、肥大细胞和少量单核和多形核白细胞。数量最多的细胞类型是成纤维细胞。人类牙龈成纤维细胞（HGFs）产生胶原蛋白、蛋白聚糖和弹性蛋白等结缔组织物质，并基本上维持牙龈固有层的完整性。本研究观察了人牙龈成纤维细胞条件培养液（HGF-CM）对人牙周膜成纤维细胞（HPLFs）、人牙槽骨细胞（HABCs）和人牙龈上皮细胞（HGEs）趋化性的影响，以确定HGF-CM对成纤维细胞是否具有趋化性和/或趋化动力学作用。进行Zigmond-Hirsch型的棋盘分析，其中将不同浓度的HGF-CM加入到改进的盲孔Boyden型室的上部（细胞）和下部（测试）隔室中，并测量成纤维细胞的迁移。这一分析表明，HGF-CM诱导的HGF和HPLF的趋化迁移，因为细胞迁移增加的存在下，正梯度，即175 ng/ml浓度的肽在较低的隔室比在较高的隔室。在高于50% HGF-CM的浓度下，HGF-CM一致地增加HABC和HGE的迁移，与作为阳性对照的HPLF-CM（100%）的结果相似。提示HGF-CM可能有助于牙周组织再生。

At the completion of flap reflection, the soft tissues are placed in contact with the root surface. Epithelial and connective tissue reattachment is complete at 3 weeks. Freely mobile cells of the gingival lamina propria include fibroblast, mast cells, and a small number of mononuclear and polymorphonuclear leukocytes. The most numerous cell type is the fibroblast. Human gingival fibroblasts (HGFs) produce connective tissue substances including collagen, , proteoglycans, and elastin, and essentially maintain the integrity of gingival lamina propria. The present investigation was conducted evaluate the effect of human gingival fibroblasts conditioned medium (HGF-CM) to chemotaxis of human periodontal ligament fibroblasts (HPLFs), HGF and human alveolar bone cells (HABCs), and human gingival epithelial cells (HGEs).To determine whether HGF-CM was chemotactic, and/or chemokinetic for fibroblast, a checcurboard analysis of the Zigmond-Hirsch type was performed wherein different concentrations of HGF-CM were added to upper (cell) and lower (test) compartment of modified blind-well Boyden-type chamber, and migration of fibroblasts was measured. This analysis suggests that HGF-CM induced chemotactic migration of HGF and HPLF,because the cells migration increased in the presence of positive gradient, i.e.a higher of 175ng/ml concentration of the peptide in the lower compartment than in the upper compartment. HGF-CM consistently increased migration of HABC and HGE at concentration higher of 50% HGF-CM,similar result of HPLF-CM (100%) which was positive control. The data suggested that HGF-CM may contribute to the development of periodontal regeneration.