Identification of markers of flavonoid action in colonic cancer cells

结肠癌细胞中类黄酮作用标记物的鉴定

• 批准号: 5391242
• 负责人: Professor Dr. Uwe Wenzel
• 金额: --
• 依托单位: Professur für Biochemie und Molekularbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5391242?language=en
• 关键词: Identification; markers; flavonoid; action; colonic

The analysis of the proteome as the protein complement of the genome of a cell or organ allows to define the molecular differences between normal cells and transformed cells in the process of carcinogenesis and helps to identify the molecular targets of compounds that alter the transformation process. We have shown recently that flavone, a flavonoid occuring in a variety of plants induces potently apoptosis in colon cancer cell lines such as H-29, whereas non-transformed colonocytes were not affected by the compound. We now would like to use differential display proteome analysis of transformed colonocytes (HT-20) and non-transformed colonocytes (NCOL-1) for identification of the highly selective adaptative changes in protein expression by treatment of the cells with flavone. Although a few genes with changed expression after flavone treatment of cells have been identified by semi-quantitative PCR and Western-blot analysis, a comprehensive analysis of target protein expression is important for the understanding of the mode of action based on different genetic backgrounds and in few of its possible side effects. In addition, the project may help to identify more specific biomarkes for diagnosis or interventional programs in colonic carcinogenesis.

蛋白质组作为细胞或器官基因组的蛋白质补充物的分析允许定义在致癌过程中正常细胞和转化细胞之间的分子差异，并有助于鉴定改变转化过程的化合物的分子靶标。我们最近发现，黄酮类化合物，一种存在于多种植物中的黄酮类化合物，在结肠癌细胞系如H-29中诱导有效的凋亡，而非转化的结肠细胞不受该化合物的影响。我们现在想使用差异显示蛋白质组分析的转化的结肠细胞（HT-20）和非转化的结肠细胞（NCOL-1）的蛋白质表达的高度选择性的适应性变化，通过处理的细胞与黄酮的鉴定。虽然已经通过半定量PCR和Western-blot分析鉴定了黄酮处理细胞后表达改变的一些基因，但是对靶蛋白表达的全面分析对于理解基于不同遗传背景的作用模式以及其可能的副作用很少是重要的。此外，该项目可能有助于确定更具体的生物标记物，用于结肠癌发生的诊断或干预计划。