Analysis for the mechanism of petal growth during flower opening and control for the cut flower opening in post-harvest conditions

花期花瓣生长机制分析及采后切花开放控制

• 批准号: 21688002
• 负责人: YAMADA Kunio
• 金额: $ 14.89万
• 依托单位: Chubu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21688002/
• 关键词: 園芸学; バラ; ポストハーベスト; 細胞壁; エクスパンシン; XTH; トルコギキョウ; ポストハーベス

The aims of this project are to clarify the mechanism of petal growth during flower opening, and to control flower opening of cut roses in post-harvest conditions. I found that the Expansin and XTH play important roles for flower opening of cut roses by real-time PCR analysis and western blot analysis. And XG9 was analyzed as an inhibitor for XTH enzyme activity. XG9 promoted the flower opening of cut roses at high concentration, and in contrast, XG9 inhibited it at low concentration. Furthermore, it became clear that the petal of cut rose buds grew for only a few hours from the initiation of light period, and it hardly grew during the rest periods.

本研究的目的在于阐明玫瑰切花开放过程中花瓣生长的机理，并在采后条件下控制切花的开放。通过实时荧光定量PCR分析和Western blot分析，发现Expansin和XTH在月季切花开放过程中起重要作用。XG9作为XTH酶活性的抑制剂进行了分析。高浓度XG9促进切花月季的开放，低浓度XG9抑制切花月季的开放。此外，很明显，切割玫瑰花蕾的花瓣从光照期开始仅生长几个小时，并且在休息期间几乎不生长。

项目成果

Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening

• DOI: 10.2503/jjshs1.78.242
• 发表时间: 2009-04
• 期刊: Journal of The Japanese Society for Horticultural Science
• 作者: K. Yamada;R. Takahashi;C. Fujitani;Keiko Mishima;Masato Yoshida;D. Joyce;S. Yamaki

細胞伸長阻害剤による花弁成長制御

通过细胞伸长抑制剂控制花瓣生长

• 发表时间: 2009
• 作者: 白武勝裕;他;白武勝裕;白武勝裕;堀部貴紀;高垣奈保
• 通讯作者: 高垣奈保

Role of expansin and XTH duirng rose flower opening

扩张蛋白和 XTH 在玫瑰花开放过程中的作用

• 发表时间: 2009
• 作者: Ochiai;M.;Takagaki;N.;Maesaka;M. and Yamada;K.
• 通讯作者: K.

トルコギキョウ花弁におけるエクスパンシンおよびエンド型キシログルカン転移酵素/加水分解酵素(XTH)の解析

洋桔梗花瓣中扩展蛋白和内型木葡聚糖转移酶/水解酶 (XTH) 的分析

• 发表时间: 2009
• 作者: 白武勝裕;他;白武勝裕;白武勝裕;堀部貴紀;高垣奈保;落合正樹
• 通讯作者: 落合正樹

Structural characteristics of SLF/SFB-genes in apple cultivars with similar S-RNase sequences

具有相似S-RNase序列的苹果品种SLF/SFB基因的结构特征

• 发表时间: 2012
• 期刊: Acta Horticulturae
• 作者: Hayashi;H.;Yamada;K.;Shiratake;K. and Matsumoto;S.
• 通讯作者: S.