Analysis for the mechanism of petal growth during flower opening and control for the cut flower opening in post-harvest conditions

本项目旨在阐明月季切花过程中花瓣生长的机理，并控制切花月季在采后条件下的花朵开放。通过实时荧光定量聚合酶链式反应和蛋白质印迹分析，发现Expansin和Xth在月季切花开放过程中起重要作用。XG9是Xth酶活性的抑制因子。XG9在高浓度时促进切花月季的开放，而在低浓度时则抑制其开放。此外，切花月季花蕾的花瓣从发光期开始只生长了几个小时，而在休止期几乎没有生长。

The aims of this project are to clarify the mechanism of petal growth during flower opening, and to control flower opening of cut roses in post-harvest conditions. I found that the Expansin and XTH play important roles for flower opening of cut roses by real-time PCR analysis and western blot analysis. And XG9 was analyzed as an inhibitor for XTH enzyme activity. XG9 promoted the flower opening of cut roses at high concentration, and in contrast, XG9 inhibited it at low concentration. Furthermore, it became clear that the petal of cut rose buds grew for only a few hours from the initiation of light period, and it hardly grew during the rest periods.