Analysis for the mechanism of petal growth during flower opening and control for the cut flower opening in post-harvest conditions

本课题旨在阐明切花玫瑰开花过程中花瓣生长的机理，并在采后条件下控制切花玫瑰的开花。通过实时荧光定量PCR和western blot分析，我发现Expansin和XTH在切花玫瑰开花过程中起重要作用。并分析了XG9作为XTH酶活性抑制剂的作用。高浓度XG9对切花玫瑰开花有促进作用，低浓度XG9对切花玫瑰开花有抑制作用。此外，我们还清楚地发现，切花玫瑰花蕾的花瓣在光照期开始后只生长了几个小时，在其他时期几乎没有生长。

The aims of this project are to clarify the mechanism of petal growth during flower opening, and to control flower opening of cut roses in post-harvest conditions. I found that the Expansin and XTH play important roles for flower opening of cut roses by real-time PCR analysis and western blot analysis. And XG9 was analyzed as an inhibitor for XTH enzyme activity. XG9 promoted the flower opening of cut roses at high concentration, and in contrast, XG9 inhibited it at low concentration. Furthermore, it became clear that the petal of cut rose buds grew for only a few hours from the initiation of light period, and it hardly grew during the rest periods.