Analysis for the mechanism of petal growth during flower opening and control for the cut flower opening in post-harvest conditions

本研究的目的在于阐明玫瑰切花开放过程中花瓣生长的机理，并在采后条件下控制切花的开放。通过实时荧光定量PCR分析和Western blot分析，发现Expansin和XTH在月季切花开放过程中起重要作用。XG9作为XTH酶活性的抑制剂进行了分析。高浓度XG9促进切花月季的开放，低浓度XG9抑制切花月季的开放。此外，很明显，切割玫瑰花蕾的花瓣从光照期开始仅生长几个小时，并且在休息期间几乎不生长。

The aims of this project are to clarify the mechanism of petal growth during flower opening, and to control flower opening of cut roses in post-harvest conditions. I found that the Expansin and XTH play important roles for flower opening of cut roses by real-time PCR analysis and western blot analysis. And XG9 was analyzed as an inhibitor for XTH enzyme activity. XG9 promoted the flower opening of cut roses at high concentration, and in contrast, XG9 inhibited it at low concentration. Furthermore, it became clear that the petal of cut rose buds grew for only a few hours from the initiation of light period, and it hardly grew during the rest periods.