Fundamental research for development of new therapy for canine lymphoma

犬淋巴瘤新疗法开发的基础研究

• 批准号: 21688022
• 负责人: MIZUNO Takuya
• 金额: $ 15.72万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21688022/
• 关键词: 犬; リンパ腫; 新規治療法

Using hybridoma library established from rat immunized with canine lymphoma cell line, canine PSGL-1 was found to be expressed on normal lymphocytes, but was specifically glycosylated in lymphoma cell line. This indicated that specifically glycosylated canine PSGL-1 molecule could be a target for antibody therapy. On the other hands, Flavopiridol, known as pan-CDK inhibitor, inhibited growth of eight lymphoma cell lines in vitro by regulation of cell cycle, as well as downregulation of anti-apoptotic proteins. Furthermore, we identified the genes specifically expressed in canine lymphoma, which could be a candidate for molecular target therapy in canine lymphoma.

利用犬淋巴瘤细胞系免疫大鼠建立的杂交瘤文库，发现犬PSGL-1在正常淋巴细胞上表达，但在淋巴瘤细胞系中特异性糖基化。这表明特异性糖基化的犬PSGL-1分子可能成为抗体治疗的靶点。另一方面，黄匹吡醇作为泛cdk抑制剂，在体外通过调控细胞周期和下调抗凋亡蛋白抑制8种淋巴瘤细胞系的生长。此外，我们还发现了在犬淋巴瘤中特异性表达的基因，这可能是犬淋巴瘤分子靶向治疗的候选基因。

项目成果

Characterization of monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (PSGL-1)

• DOI: 10.1016/j.vetimm.2011.04.009
• 发表时间: 2011-07-15
• 期刊: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
• 影响因子: 1.8
• 作者: Umeki, Saori;Suzuki, Ryoichi;Mizuno, Takuya
• 通讯作者: Mizuno, Takuya

Calreticulin expression in neoplastic versus normal dog mammary glands: A cDNA subtraction-based study

• DOI: 10.1016/j.rvsc.2010.10.028
• 发表时间: 2012-02-01
• 期刊: RESEARCH IN VETERINARY SCIENCE
• 影响因子: 2.4
• 作者: Okawa, Takumi;Kurio, Yuzo;Mizuno, Takuya
• 通讯作者: Mizuno, Takuya

Neutropenia associated with osteomyelitis due to Hepatozoon canis infection in a dog.

犬肝虫感染导致的中性粒细胞减少症与骨髓炎相关。

• DOI: 10.1292/jvms.11-0202
• 发表时间: 2011
• 期刊: The Journal of veterinary medical science
• 作者: Takako Shimokawa Miyama;S. Umeki;K. Baba;K. Sada;H. Hiraoka;Y. Endo;H. Inokuma;M. Hisasue;M. Okuda;T. Mizuno
• 通讯作者: T. Mizuno

イヌにおける癌関連遺伝子WT-1の発現の解析

犬癌症相关基因WT-1的表达分析

• 发表时间: 2011
• 作者: 酒井治;栗尾雄三;梅城沙織;酒井洋樹;久保正仁;森本将弘;林俊春;平岡博子;奥田優;水野拓也
• 通讯作者: 水野拓也

水野拓也サイクリン依存性キナーゼ(Cdk)阻害剤を用いたイヌのリンパ腫腫瘍細胞株の増殖抑制のメカニズムの解析

Takuy​​a Mizuno 使用细胞周期蛋白依赖性激酶（Cdk）抑制剂分析犬淋巴瘤肿瘤细胞系生长抑制机制

• 发表时间: 2011
• 作者: 江馬康夫;梅城沙織;奥田優
• 通讯作者: 奥田優