Is "steric hindrance effect" the possible new concept for tumor metastasis mechanism?

“空间位阻效应”是否可能是肿瘤转移机制的新概念？

• 批准号: 24658268
• 负责人: MIZUNO Takuya
• 金额: $ 2.58万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658268/
• 关键词: PSGL-1; 犬; 腫瘍

The effect of PSGL-1 expression on tumor cells on tumor metastasis was examined. Canine PSGL-1 was not detected in naturally occurred canine solid tumor by immunohistochemistry, but was detected in canine lymphoma samples. Injection of canine lymphoma cell lines which has lower level of PSGL1 by knockdown technique into NOJ mice had lower rate of metastasis as compared with parental lymphoma cell lines. Canine mammary gland tumor cell lines overexrpressing PSGL-1 into NOD/SCID mice appeared to have more lung metastasis. In conclusion, this study indicated that PSGL-1 has an important role in tumor metastasis or dissemination.

观察肿瘤细胞中PSGL-1表达对肿瘤转移的影响。犬PSGL-1免疫组化未在自然发生的犬实体瘤中检测到，但在犬淋巴瘤样本中检测到。将低水平PSGL1基因敲低的犬淋巴瘤细胞株注射到NOJ小鼠体内，与亲本淋巴瘤细胞株相比，转移率较低。过表达PSGL-1的犬乳腺肿瘤细胞系在NOD/SCID小鼠中表现出更多的肺转移。总之，本研究提示PSGL-1在肿瘤转移或传播中具有重要作用。

项目成果

Reovirus type-2 infection in newborn DBA/1J mice reduces the development of late allergic asthma

新生 DBA/1J 小鼠的 2 型呼肠孤病毒感染可减少晚期过敏性哮喘的发展

• 发表时间: 2012
• 期刊: Int J Exp Patho
• 作者: Nakashima T;Hayashi T;Mizuno T
• 通讯作者: Mizuno T

Vaccination with Antigen-Transfected, NKT Cell Ligand-Loaded, Human Cells Elicits Robust In Situ Immune Responses by Dendritic Cells

• DOI: 10.1158/0008-5472.can-12-0759
• 发表时间: 2013-01-01
• 期刊: CANCER RESEARCH
• 影响因子: 11.2
• 作者: Shimizu, Kanako;Mizuno, Takuya;Fujii, Shin-ichiro
• 通讯作者: Fujii, Shin-ichiro

Oncolytic reovirus in canine mast cell tumor.

• DOI: 10.1371/journal.pone.0073555
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Hwang CC;Umeki S;Kubo M;Hayashi T;Shimoda H;Mochizuki M;Maeda K;Baba K;Hiraoka H;Coffey M;Okuda M;Mizuno T
• 通讯作者: Mizuno T

レオウイルスによる犬の各種腫瘍細胞株に対する細胞死の誘導

呼肠孤病毒诱导多种犬肿瘤细胞系细胞死亡

• 发表时间: 2013
• 作者: 伊賀瀬雅也;Chung Chew Hwang;奥田優;水野拓也
• 通讯作者: 水野拓也

新しく樹立したイヌリンパ腫細胞株のin vitroおよびin vivoにおける性状解析

新建立的犬淋巴瘤细胞系的体外和体内表征

• 发表时间: 2012
• 作者: 梅城沙織;江馬康夫;鈴木綾一;久保正仁,林俊春;岡村泰彦;山崎淳平;辻本元;谷健二;平岡博子;奥田優;水野拓也
• 通讯作者: 水野拓也