Molecular mechanisms of retinal axon branching and synaptogenesis

视网膜轴突分支和突触发生的分子机制

• 批准号: 21700364
• 负责人: SUZUKI Ryoko
• 金额: $ 2.33万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700364/
• 关键词: 神経回路形成; 軸索側枝; 神経栄養因子; ニワトリ

SPIG1 is a secretory molecule of unknown function, which is composed of a follistatin-like domain, an extracellular calcium-binding domain, and two immunoglobulin-like domains. I have here investigated the role of SIPIG1 during the branch formation, elaboration of axon terminals and synaptic formation in retinal axons. I found that SIPIG1 interacts with one of a neurotrophic receptor. I also investigated the possibility that SPIG1 is involved in synaptic formation during development. In SPIG1 knock-down dorsalretina, the superficial layers of the optic tectum were thin. However, the synapse structure and synaptic positions were normal. These results indicate that SPIG1 is involved in branch formation and refinement of developing retinal axons, and also SPIG1 might function as an axonally secreted regulator of the local extracellular proteolysis involved in the innervation by retinal axons within the tectum.

SPIG1是一个未知功能的分泌分子，由卵泡素样结构域，一个细胞外钙结合结构域和两个免疫球蛋白样结构域组成。我在这里研究了SIPIG1在分支形成，轴突末端的阐述和视网膜轴突中突触形成的作用。我发现SIPIG1与神经营养受体之一相互作用。我还调查了SPIG1在开发过程中参与突触形成的可能性。在SPIG1敲除多边形的后核中，视觉底面的浅层层很薄。但是，突触结构和突触位置正常。这些结果表明，SPIG1参与了分支形成和发育中的视网膜轴突的完善，并且SPIG1也可能是局部细胞外蛋白水解的轴突分泌的调节剂，参与构件内视网膜轴突的神经支配。