Molecular mechanisms of retinal axon branching and synaptogenesis

视网膜轴突分支和突触发生的分子机制

• 批准号: 21700364
• 负责人: SUZUKI Ryoko
• 金额: $ 2.33万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700364/
• 关键词: 神経回路形成; 軸索側枝; 神経栄養因子; ニワトリ

SPIG1 is a secretory molecule of unknown function, which is composed of a follistatin-like domain, an extracellular calcium-binding domain, and two immunoglobulin-like domains. I have here investigated the role of SIPIG1 during the branch formation, elaboration of axon terminals and synaptic formation in retinal axons. I found that SIPIG1 interacts with one of a neurotrophic receptor. I also investigated the possibility that SPIG1 is involved in synaptic formation during development. In SPIG1 knock-down dorsalretina, the superficial layers of the optic tectum were thin. However, the synapse structure and synaptic positions were normal. These results indicate that SPIG1 is involved in branch formation and refinement of developing retinal axons, and also SPIG1 might function as an axonally secreted regulator of the local extracellular proteolysis involved in the innervation by retinal axons within the tectum.

SPIG1是一种功能未知的分泌分子，由一个卵泡抑素样结构域、一个细胞外钙结合结构域和两个免疫球蛋白样结构域组成。我在这里研究了 SIPIG1 在分支形成、轴突末端的细化和视网膜轴突突触形成过程中的作用。我发现 SIPIG1 与一种神经营养受体相互作用。我还研究了 SPIG1 参与发育过程中突触形成的可能性。在 SPIG1 敲除的背视网膜中，视顶盖的表层很薄。然而，突触结构和突触位置正常。这些结果表明 SPIG1 参与发育中的视网膜轴突的分支形成和细化，并且 SPIG1 也可能作为局部细胞外蛋白水解的轴突分泌调节剂，参与顶盖内视网膜轴突的神经支配。