Study on the mechanism of the differentiation control of osteoblast and osteoclast based on the surface characteristics of biomaterials for hard tissues

本研究旨在研究极化羟基磷灰石（HA）的表面特性及极化电刺激对体外培养成骨细胞行为的影响，探讨其促进骨传导的机制。基于材料分析，表面特性表明，电极化对表面粗糙度，结晶度和组成元素没有影响。根据接触角的测量，电极化的HA，它提供了两种类型的表面，带负电荷的HA（N-HA）和带正电荷的HA（P-HA），甚至比正常的HA更亲水。形态学观察和定量分析表明，典型的粘附细胞在HA上呈圆形，而在N-HA和P-HA上呈纺锤形或扇形铺展。肌动蛋白染色阳性的细胞面积，这表明细胞的扩展程度，明显大于在N-HA和P-HA上的HA。总之，电极化改善了HA表面的润湿性，并影响成骨细胞的粘附，尤其是扩散。

The purpose of this study was to study the surface characteristics of polarized hydroxyapatite (HA) and the effects of electrical stimulation by polarization on osteoblast behavior in vitro in order to investigate the mechanism of the osteoconductive enhancement. Based on the material analysis, the surface characteristics revealed that electrical polarization had no effect on surface roughness, crystallinity, and constituent elements. According to contact angle measurement, electrically polarized HA, which provides two kinds of surfaces, negatively charged HA (N-HA) and positively charged HA (P-HA), was even more hydrophilic than that of normal HA. Morphological observations and quantitative analyses revealed that the typical adhered cells had a round shape on the HA but had a spindle or fan-like spreading configuration on the N-HA and the P-HA. The cell areas positively stained for actin, which indicates the degree of cell spreading, were distinctly larger on the N-HA and P-HA than that on the HA. In conclusion, electrical polarization improved the wettability of the HA surface and affected osteoblastic adhesion, especially spreading.