Demonstration of persistent activation of ATM on translocated chromosomes by Foci on FISH method.

通过 FISH 方法的 Foci 演示易位染色体上 ATM 的持续激活。

• 批准号: 21710061
• 负责人: YAMAUCHI Motohiro
• 金额: $ 2.83万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21710061/
• 关键词: 応答; ATM; 染色体転座; γ-H2AX; 細胞周期チェックポイント; DNA二重鎖切断; H2AX; フォーカス; FISH

I established" Foci on FISH" method, which combines immunofluorescence staining of phosphorylated histone H2AX and fluorescence in situ hybridization(FISH) of chromosomes. Using this technique, I demonstrated that H2AX phosphorylation, which indicates ATM activation, persists on dicentric chromosomes, a kind of chromosome translocation. Moreover, the present study revealed that ATM suppresses frequency of ionizing radiation-induced chromosome translocation via p53-dependent cell cycle checkpoint and DNA-PK-dependent DNA double-strand break repair pathway.

建立了磷酸化组蛋白H2 AX免疫荧光染色与染色体荧光原位杂交相结合的”Foci on FISH”方法。使用这种技术，我证明了H2 AX磷酸化，这表明ATM激活，坚持双着丝粒染色体，一种染色体易位。此外，本研究表明，ATM抑制电离辐射诱导的染色体易位的频率通过p53依赖的细胞周期检查点和DNA-PK依赖的DNA双链断裂修复途径。

项目成果

Creating localized DNA double-strand breaks with microirradiation

利用微辐射产生局部 DNA 双链断裂

• 发表时间: 2011
• 期刊: Nature Protocol
• 作者: Suzuki K;Yamauchi M;Oka Y;Suzuki M;Yamashita S.
• 通讯作者: Yamashita S.

Image-based quantitative determination of DNA damage signal reveals a threshold for G2 checkpoint activation in response to ionizing radiation.

• DOI: 10.1186/2041-9414-1-10
• 发表时间: 2010-08-04
• 期刊: Genome integrity
• 作者: Ishikawa A;Yamauchi M;Suzuki K;Yamashita S
• 通讯作者: Yamashita S

誤ったDNA二重鎖切断再結合部位に対するATM依存的チェックポイント応答

ATM 依赖性检查点对错误 DNA 双链断裂重组位点的反应

• 发表时间: 2010
• 作者: K.Zhumadilov;A.Ivannikov;D.Zharlyganova;I.Shashtin;Zh.Zhumadilov;S.Toyoda;S.Endo;M.Hoshi.;山内基弘
• 通讯作者: 山内基弘

Mode of ATM-dependent suppression of chromosome translocation.

ATM 依赖性染色体易位抑制模式。

• DOI: 10.1016/j.bbrc.2011.11.006
• 发表时间: 2011
• 期刊: Biochem Biophys Res Commun
• 作者: Yamauchi M;Suzuki K;Oka Y;Suzuki M;Kondo H;Yamashita S
• 通讯作者: Yamashita S

Microbeam Irradiation Facilities for Radiobiology in Japan and China

• DOI: 10.1269/jrr.09009s
• 发表时间: 2009-03-01
• 期刊: JOURNAL OF RADIATION RESEARCH
• 影响因子: 2
• 作者: Kobayashi, Yasuhiko;Funayama, Tomoo;Wu, Lijun
• 通讯作者: Wu, Lijun