Demonstration of persistent activation of ATM on translocated chromosomes by Foci on FISH method.

点击翻译按钮获取中文摘要

I established" Foci on FISH" method, which combines immunofluorescence staining of phosphorylated histone H2AX and fluorescence in situ hybridization(FISH) of chromosomes. Using this technique, I demonstrated that H2AX phosphorylation, which indicates ATM activation, persists on dicentric chromosomes, a kind of chromosome translocation. Moreover, the present study revealed that ATM suppresses frequency of ionizing radiation-induced chromosome translocation via p53-dependent cell cycle checkpoint and DNA-PK-dependent DNA double-strand break repair pathway.