Analysis of transcriptional regulation by Cohesin

点击翻译按钮获取中文摘要

I have developed a system to deplete SMC3, one of essential cohesin subunits, specifically in the neuron cells of mice. The neuron could not survive and cell death was induced. I am analyzing gene expression profile and chromatin high order structure at each stage of differentiation. Meanwhile I have identified HDAC8 as a specific deaceylase for SMC3 and causative gene for CdLS. These findings suggest that acetylation status of cohesin is also important for transcriptional regulation. I am now trying to analyze biochemical property of acetylated Smc3 and its genomic localization to understand the mechanism behind regulation of transcription by acetylation.