Biological design principle-based synthetic biology

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We define bioalgorithms as the step-by-step molecular process of how the biochemical networks including elementary networks and network motifs generate their particular functions, that is to say, the network-function relationships (NFR). The bioalgorithms would be invaluable for synthetic biology, providing an instruction for how we engineer robust biological circuits that carry out a target function. In this study, based on the bioalgorithms, we combined subnetworks of glycolysis, TCA cycle, pentose phosphate pathway, and the anapleorotic pathways to present a dynamic model of the E. call central metabolic network. Our model reproduced the changing patterns of extracellular and intracellular metabolite concentrations in the batch and continuous cultivations.