Molecular mechanisms for central circadian pacemaker regulations

中央昼夜节律起搏器调节的分子机制

• 批准号: 22300108
• 负责人: IKEDA MASAYUKI
• 金额: $ 11.48万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22300108/
• 关键词: キイロショウジョウバエ; マウス; バイオイメージング; 体内時計; 分子細胞神経科学; 分子・細胞神経科学; ショウジョウバエ

Daily rhythms in diverse activities are governed by transcriptional/translational feedback loops in clock genes. Also, it has been suggested that the circadian dynamics of intracellular signaling messengers such as Ca2+ may be critical both for the cellular physiological activities and gene transcriptional cycles. Based on these, the present study analyzed the clock gene transcriptional rhythms as well as cytosolic H+/Ca2+ in diverse models including Drosophila endocrine oscillators and SCN progenitor cells. The results demonstrated presence of clock gene oscillations but lack of apparent circadian cytosolic Ca2+ waves in these modes. Thus, the present results demonstrate cell-type specific mechanisms for the circadian rhythm generations.

各种活动的日常节律由时钟基因中的转录/翻译反馈环控制。此外，有人认为细胞内信号传导信使（例如 Ca2+）的昼夜节律动态可能对于细胞生理活动和基因转录周期都至关重要。在此基础上，本研究分析了果蝇内分泌振荡器和 SCN 祖细胞等多种模型中的时钟基因转录节律以及胞质 H+/Ca2+。结果表明，在这些模式下存在时钟基因振荡，但缺乏明显的昼夜节律胞质 Ca2+ 波。因此，目前的结果证明了昼夜节律世代的细胞类型特异性机制。

项目成果

Thermal dynamics of metabotropic glutamate receptor signaling revealed by subtraction microcalorimetric measurements on live cells

通过活细胞减法微量热测量揭示代谢型谷氨酸受体信号传导的热动力学

• DOI: 10.1038/protex.2013.061
• 发表时间: 2013
• 期刊: Protocol Exchange
• 作者: Ikeda M;Takeda M & Yoshioka T
• 通讯作者: Takeda M & Yoshioka T

カドミウムイオンによる睡眠覚醒リズムの調節と毒性発現の分子時計依存性

镉离子睡眠-觉醒节律调节和毒性表达的分子钟依赖性

• 发表时间: 2012
• 作者: 池田真行;海野雄哉;竹田真浩;矢本くるみ;竹内公平;片岡絢;藤澤千里;尾崎智也;森岡絵里;三浦伸彦
• 通讯作者: 三浦伸彦

Induction of slow-wave sleep and cancelation of REM sleep by pharmacological blocking of cerebral H1 histamine receptors in rats

通过药物阻断大鼠大脑 H1 组胺受体诱导慢波睡眠和取消快速眼动睡眠

• 发表时间: 2012
• 作者: 池田真行;海野雄哉
• 通讯作者: 海野雄哉

Functional Compensation between Cholecystokinin-1 and -2 Receptors in Murine Paraventricular Nucleus Neurons*

• DOI: 10.1074/jbc.m112.416214
• 发表时间: 2012-10
• 期刊: The Journal of Biological Chemistry
• 作者: Shahid Mohammad;Tomoya Ozaki;Kouhei Takeuchi;K. Unno;Kurumi Yamoto;Eri Morioka;S. Takiguchi;M. Ikeda

Induction of prolonged, continuous slow-wave sleep by blocking cerebral H1 histamine receptors in rats

• DOI: 10.1111/j.1476-5381.2011.01547.x
• 发表时间: 2012-01-01
• 期刊: BRITISH JOURNAL OF PHARMACOLOGY
• 影响因子: 7.3
• 作者: Ikeda-Sagara, Masami;Ozaki, Tomoya;Ikeda, Masayuki
• 通讯作者: Ikeda, Masayuki