Development of multi-lateral cancer gene therapy by enhancing anti-tumor activity of inactivated Sendai virus particle

通过增强灭活仙台病毒颗粒的抗肿瘤活性开发多方癌症基因疗法

• 批准号: 22300339
• 负责人: KANEDA Yasufumi
• 金额: $ 11.15万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22300339/
• 关键词: HVJ; 癌; 抗腫瘍免疫; アポトーシス; 遺伝子解析; 遺伝子治療; 核酸医薬

We have reported that UV-inactivated Sendai virus particles (hemagglutinating virus of Japan envelope; HVJ-E) have multiple anti-tumor activities. Clinical application of HVJ-E has been started to evaluate the safety and efficacy of the particle in cancer patients. To enhance the anti-tumor activities, we attempted to incorporate therapeutic molecules into HVJ-E. As a result, HVJ-E containing IL2 or IL-12 gene enhanced anti-tumor immunity. HVJ-E having IL-12 polypeptide on the surface was developed and was more effective for the treatment of melanoma than original HVJ-E alone. Rad51 siRNA incorporated into HVJ-E augmented the effect of cancer treatment when combined with anti-tumor reagent, dacarbazine.

我们已经报道了紫外线灭活仙台病毒颗粒（日本包膜血凝病毒; HVJ-E）具有多种抗肿瘤活性。HVJ-E已开始临床应用，以评估该颗粒在癌症患者中的安全性和有效性。为了增强抗肿瘤活性，我们尝试将治疗性分子并入HVJ-E中。结果表明，含有IL-2或IL-12基因的HVJ-E增强了抗肿瘤免疫。开发了在表面上具有IL-12多肽的HVJ-E，并且其对于黑素瘤的治疗比单独的原始HVJ-E更有效。当与抗肿瘤试剂达卡巴嗪组合时，掺入HVJ-E中的Rad 51 siRNA增强了癌症治疗的效果。

项目成果

In vitro investigation of efficient photodynamic therapy using a nonviral vector; hemagglutinating virus of Japan envelope.

• DOI: 10.1117/1.jbo.17.7.078002
• 发表时间: 2012-07
• 期刊: Journal of biomedical optics
• 影响因子: 3.5
• 作者: M. Sakai;N. Fujimoto;K. Ishii;Hiroyuki Nakamura;Y. Kaneda;K. Awazu

新規抗がん剤としての不活化ウイルス粒子のポテンシャル

灭活病毒颗粒作为新型抗癌剂的潜力

• 发表时间: 2012
• 作者: 中川晋作;吉川 舞;金田安史
• 通讯作者: 金田安史

Systemic administration of a novel immune-stimulatory pseudovirion suppresses lung metastatic melanoma by regionally enhancing IFN-gamma production

全身施用新型免疫刺激性假病毒粒子通过局部增强 IFN-γ 产生来抑制肺转移性黑色素瘤

• 发表时间: 2012
• 期刊: Clinical Cancer Research
• 影响因子: 11.5
• 作者: 西村孝司;小林稔;脇田大功;北村秀光;芦野滋;Ishida T;Saga K.
• 通讯作者: Saga K.

Methyl-beta cyclodextrin alters the production and infectivity of Sendai virus.

甲基-β环糊精改变仙台病毒的产生和感染性。

• 发表时间: 2011
• 期刊: Arch Virol.
• 作者: Fujita H;Tamai K;Kawachi M;Saga K;Shimbo T;Yamazaki T;Kaneda Y.
• 通讯作者: Kaneda Y.

Immunogene therapy using immunomodulating HVJ-E vector augments and-tumor effects in murine malignant glioma.

使用免疫调节 HVJ-E 载体的免疫基因治疗可增强小鼠恶性神经胶质瘤的肿瘤效应。

• 发表时间: 2011
• 期刊: J.Neuro-Oncology
• 作者: Matsuda;M.;Nimura;K.;Shimbo;T.;Hamasaki;T.;Yamamoto;T.;Matsumura;A.;Kaneda;Y.
• 通讯作者: Y.