Development of microspectroscopic method measuring faint absorbance of transition metal complexes existing at liquid/liquid interface and evaluation of their interfacial functions

在本研究中，我们主要开发了以下两种方法：1 .高灵敏度显微镜图像小区域的亮度测量；使用具有超高灵敏度的偏振光测量小区域的亮度。这些方法使我们能够测量在十二烷-甲苯/水界面形成的四苯基卟啉锰（III）单分子层的微弱吸光度和吸收光谱，以及属于原生动物的法氏草草虫和绿藻中叶绿素a和b的微弱吸光度和吸收光谱。

In the present study, we mainly developed the following two methods, 1. measurements of brightness of small regions of summarized microscope images with a high sensitivity, 2. measurements of brightness of small regions using polarized light with a ultrahigh sensitivity. These methods enabled us to measure faint absorbances and absorption spectra of monolayers of tetraphenylporphinatomanganese(III) formed at the dodecane-toluene/water interface and those of chlorophyll a and b in Paramecium bursaria and Euglena, which belong to protozoa.