Multistage dielectrophoretic separation of various kinds of biological microparticles with quadrupole microelectrode capillary

四极微电极毛细管多级介电泳分离各类生物微粒

• 批准号: 13554032
• 负责人: TSUKAHARA Satoshi
• 金额: $ 9.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13554032/
• 关键词: Dielectrophoresis; Fluorescence Microscope; Field flow fractionation; Planar quadrupole electrode; Biological microparticles; Lectin protein; Concanavalin A; Yeast cell; デキストラン

Dielectrophoresis of single microparticles (0.35 - 7.3 μm in radius) and DNA (40 kbp) were measured in a planar quadrupole microelectrode (50 μm or 65 μm in working area radius) with a fluorescence microscope. An alternating current (ac) of 1 kHz - 10 MHz in frequency and 0 - 5 V in voltage was applied to the quadrupole microelectrode. Polystyrene, polystyrene-carboxylate and carbon microparticles, and yeast cell were adopted as a sample microparticle. The dielectrophoretic mobilities of the single microparticles and single DNA were analyzed quantitatively with their intrinsic and surface conductivities, their permittivity, and their size as well as the conductivity and permittivity of aqueous media. Some separations among the microparticles were demonstrated with a fabricated capillary quadrupole microelectrode (82.5 μm in radius of working area, 2.35 cm in length) by combining the technique of field flow fractionation.

用荧光显微镜在平面四极微电极（工作区半径为50 μm或65 μm）上测量了单个微粒（半径为0.35 - 7.3 μm）和DNA（40 kbp）的介电电泳。将频率为1 kHz - 10 MHz和电压为0 - 5 V的交流电（ac）施加到四极微电极。采用聚苯乙烯、聚苯乙烯-羧酸酯和碳微粒以及酵母细胞作为样品微粒。通过其固有电导率、表面电导率、介电常数、尺寸以及水介质的电导率和介电常数，定量分析了单个微粒和单个DNA的介电电泳迁移率。采用自制的毛细管四极微电极（工作区半径82.5 μm，长2.35cm），结合场流分离技术，实现了微粒间的分离。

项目成果

TSUKAHARA, Satoshi: "Flow Fractionation of Microparticles under Dielectrophoreti Field in Quadrupole Electrode Capillary"Anal.Chem.. 73(23). 5661-5668 (2001)

冢原聪：“四极电极毛细管介电泳场下微粒的流动分级”Anal.Chem.. 73(23)。

池田育浩: "レーザーアブレーション法による誘電泳動用平面マイクロ多重極電極の作製"分析化学. 51(9). 767-773 (2002)

Ikuhiro Ikeda：“使用激光烧蚀方法制备用于介电泳的平面微多极电极”分析化学 51(9) 767-773 (2002)。

TSUKAHARA,Satoshi: "Dielectrophoretic Behavior of Single DNA in Planar and Capillary Quadrupole Microelectrodes"Chem. Lett.. 250-251 (2001)

冢原聪：“平面和毛细管四极微电极中单个 DNA 的介电泳行为”Chem。

TSUKAHARA,Satoshi: "Flow Fractionation of Microparticles under Dielectrophoretic Field in Quadrupole Electrode Capillary"Anal. Chem.. 73(23). 5661-5668 (2001)

冢原聪：“四极电极毛细管介电泳场下微粒的流动分离”分析。

IKEDA,Ikuhiro: "Effects of Viability and Lectin Protein Binding on Dielectrophoretic Behavior of Single Yeast Cells"Anal. Sci.. 19(1). 27-31 (2003)

IKEDA，Ikuhiro：“活力和凝集素蛋白结合对单个酵母细胞介电泳行为的影响”分析。