Multistage dielectrophoretic separation of various kinds of biological microparticles with quadrupole microelectrode capillary
四极微电极毛细管多级介电泳分离各类生物微粒
基本信息
- 批准号:13554032
- 负责人:
- 金额:$ 9.22万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Dielectrophoresis of single microparticles (0.35 - 7.3 μm in radius) and DNA (40 kbp) were measured in a planar quadrupole microelectrode (50 μm or 65 μm in working area radius) with a fluorescence microscope. An alternating current (ac) of 1 kHz - 10 MHz in frequency and 0 - 5 V in voltage was applied to the quadrupole microelectrode. Polystyrene, polystyrene-carboxylate and carbon microparticles, and yeast cell were adopted as a sample microparticle. The dielectrophoretic mobilities of the single microparticles and single DNA were analyzed quantitatively with their intrinsic and surface conductivities, their permittivity, and their size as well as the conductivity and permittivity of aqueous media. Some separations among the microparticles were demonstrated with a fabricated capillary quadrupole microelectrode (82.5 μm in radius of working area, 2.35 cm in length) by combining the technique of field flow fractionation.
用荧光显微镜在平面四极微电极(工作区半径为50 μm或65 μm)上测量了单个微粒(半径为0.35 - 7.3 μm)和DNA(40 kbp)的介电电泳。将频率为1 kHz - 10 MHz和电压为0 - 5 V的交流电(ac)施加到四极微电极。采用聚苯乙烯、聚苯乙烯-羧酸酯和碳微粒以及酵母细胞作为样品微粒。的介电电泳迁移率的单个微粒和单个DNA进行了定量分析,其固有的和表面的电导率,其介电常数,和它们的大小,以及水介质的电导率和介电常数。采用自制的毛细管四极微电极(工作区半径82.5 μm,长2.35cm),结合场流分离技术,实现了微粒间的分离。
项目成果
期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
TSUKAHARA, Satoshi: "Flow Fractionation of Microparticles under Dielectrophoreti Field in Quadrupole Electrode Capillary"Anal.Chem.. 73(23). 5661-5668 (2001)
冢原聪:“四极电极毛细管介电泳场下微粒的流动分级”Anal.Chem.. 73(23)。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
池田育浩: "レーザーアブレーション法による誘電泳動用平面マイクロ多重極電極の作製"分析化学. 51(9). 767-773 (2002)
Ikuhiro Ikeda:“使用激光烧蚀方法制备用于介电泳的平面微多极电极”分析化学 51(9) 767-773 (2002)。
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
TSUKAHARA,Satoshi: "Dielectrophoretic Behavior of Single DNA in Planar and Capillary Quadrupole Microelectrodes"Chem. Lett.. 250-251 (2001)
冢原聪:“平面和毛细管四极微电极中单个 DNA 的介电泳行为”Chem。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
TSUKAHARA,Satoshi: "Flow Fractionation of Microparticles under Dielectrophoretic Field in Quadrupole Electrode Capillary"Anal. Chem.. 73(23). 5661-5668 (2001)
冢原聪:“四极电极毛细管介电泳场下微粒的流动分离”分析。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
IKEDA,Ikuhiro: "Effects of Viability and Lectin Protein Binding on Dielectrophoretic Behavior of Single Yeast Cells"Anal. Sci.. 19(1). 27-31 (2003)
IKEDA,Ikuhiro:“活力和凝集素蛋白结合对单个酵母细胞介电泳行为的影响”分析。
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- 影响因子:0
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TSUKAHARA Satoshi其他文献
TSUKAHARA Satoshi的其他文献
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{{ truncateString('TSUKAHARA Satoshi', 18)}}的其他基金
Development of the real-time ultrasensitive detection method for the chirality change of single DNA molecules in aqueous solutions
水溶液中单个DNA分子手性变化的实时超灵敏检测方法的开发
- 批准号:
25620113 - 财政年份:2013
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Rotational diffusion dynamics measurements of single aggregates existing at liquid/ liquid interfaces by using non-imaging light of optical microscope
利用光学显微镜非成像光测量液/液界面处单一聚集体的旋转扩散动力学
- 批准号:
22655020 - 财政年份:2010
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Development of microspectroscopic method measuring faint absorbance of transition metal complexes existing at liquid/liquid interface and evaluation of their interfacial functions
液/液界面过渡金属配合物微弱吸光度测量微光谱方法的建立及其界面功能评价
- 批准号:
22350034 - 财政年份:2010
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of in situ analytical method for depth profile of liquid/liquid interface using ultrasmall probes
超小型探针液/液界面深度剖面原位分析方法的开发
- 批准号:
19350040 - 财政年份:2007
- 资助金额:
$ 9.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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