Development of New Methods for Protein Chemical Modification

在本研究中，我们尝试开发了几种新的蛋白质修饰的化学方法。首先，我们开发了使用寡聚His标签和Ni（II）-NTA探针的互补识别对的共价蛋白质方法。该标记方法已成功应用于活细胞中标签融合蛋白的标记。第二，我们设计了高反应性的标签-探针对，包括α-螺旋型寡聚天冬氨酸标签和Zn（II）-DpaTyr探针。与先前开发的方法相比，该对显示出更高的蛋白质标记选择性，验证了我们的反应性标签-探针对的设计策略。第三，我们发现了一种新的蛋白质修饰荧光染料，这种荧光染料根据其水合状态表现出独特的双发射变化，我们期望这将是开发基于蛋白质的荧光生物传感器的有价值的荧光团

In this research, we have attempted to develop several new chemical methods for protein modification. First, we have developed the covalent protein method using the complementary recognition pair of oligo-His tag and Ni(II)-NTA probe. This labeling method was successfully applied to the labeling of the tag-fused proteins in living cells. Second, we have achieved the design of highly reactive tag-probe pair comprising a-helix type oligo-Asp tag and Zn(II)-DpaTyr probe. This pair showed the higher protein labeling selectivity compared to the previously developed method, validating the our design strategy of the reactive tag-probe pair. Third, we have found the new fluorescent dye for protein modification This fluorescent dye displayed a unique dual-emission change depending on its hydration state, which we expect would be valuable fluorohore to develop protein-based fluorescence biosensors