Development of Fluorescent Probe for Visualization of Intracellular Chloride Anion

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We have developed a new acridinium amide-type fluorescent sensor for chloride anion. This probe showed a large fluorescent-off signal in response to chloride anion based on the collisional quenching mechanism in the biological relevantconcentration range (from 1 mM to 100 mM). Taking advantage of the sensing ability, we successfully applied this probe to the fluorescent bioimaging of intracellular chloride anion in living HEK293 cells.We have also developed another type of acridinium amide probe that can penetrate cell membrane, which we expect would be a valuable molecular tool for analysis of biological roles of chloride anion.