Developments of Fluorescent Probes for Elucidation of Biological Functions of Phosphate Species

阐明磷酸盐生物功能的荧光探针的进展

• 批准号: 19350085
• 负责人: OJIDA Akio
• 金额: $ 9.32万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19350085/
• 关键词: 蛍光プローブ; バイオセンサー; バイオイメージング; リン酸アニオン; タンパク質; FRET; レシオ検出; キナーゼ; 分子認識; 蛋白質

In this research project, we have developed the new fluorescent probes, which are useful for elucidation of biological functions of various biological phosphate species. We especially focused on the developments of the three types of the fluorescent probes, including 1) protein-based hybrid-type biosensor for phosphorylated protein/peptide, 2) molecular probe for aggregation of phosphorylated tau protein exisiting in brain of Alztheimer's disease, and 3) molecular probe for intracellular ATP with a OFF/ON-type fluorescence sensing mode. As the results, we have successfully achieved the probes with the desirable functions in each of the three themes.

在本研究项目中，我们开发了新的荧光探针，用于阐明各种生物磷酸盐的生物学功能。重点介绍了这三类荧光探针的研究进展，包括1)基于蛋白质的复合型蛋白质/多肽生物传感器，2)存在于阿尔茨海默病脑内的磷酸化tau蛋白聚集的分子探针，3)关闭/开启荧光传感模式的细胞内ATP分子探针。结果，我们成功地实现了三个主题中每一个主题都具有理想功能的探测。

项目成果

Turn-on Fluorescence Sensing of Nucloeside Polyphosphates Using a Xanthene-Based Zn(II)Complex Chemosensor

使用基于呫吨的 Zn(II) 复合化学传感器开启核苷多磷酸的荧光传感

• 发表时间: 2008
• 期刊: Journal of the American Chemical Society 130
• 作者: A.Ojida;I.Takashima;T.Kohira;H.Nonaka;I.Hamachi
• 通讯作者: I.Hamachi

Turn-on Fluorescence Sensing of Nucloeside Polyphosphates Using a Xanthene-Based Zn(II) Complex Chemosensor

使用基于呫吨的 Zn(II) 复合化学传感器开启核苷多磷酸的荧光传感

• 发表时间: 2008
• 期刊: J. Am. Chem. Soc. 130
• 作者: A. OJIDA;I. TAKASHIMA;T. KOHIRA;H. NONAKA;I. HAMACHI
• 通讯作者: I. HAMACHI

Molecular recognition, fluorescence sensing, and biological assay of phosphate anion derivatives using artificial Zn(II)-Dpa complexes

• DOI: 10.1039/b812374h
• 发表时间: 2009-01-01
• 期刊: CHEMICAL COMMUNICATIONS
• 影响因子: 4.9
• 作者: Sakamoto, Takashi;Ojida, Akio;Hamachi, Itaru
• 通讯作者: Hamachi, Itaru

Real-time Off/On-mode Fluorescence Assay for Enzyme Reactions Involving Nucleoside Polyphosphates by Use of a Xanthene Zn(II)-Dpa Chemosensor

使用呫吨 Zn(II)-Dpa 化学传感器对涉及核苷多磷酸的酶反应进行实时关闭/开启模式荧光测定

• 发表时间: 2008
• 期刊: Chem. Lett. 37
• 作者: T. Kohira;I. Takashima;H. Nonaka;A. Ojida;I. Hamachi
• 通讯作者: I. Hamachi

Real-time fluorescence monitoring of GSK3b-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor

使用基于 BODIPY 的 Zn(II)-Dpa 化学传感器实时荧光监测 GSK3b 催化的磷酸化

• 发表时间: 2009
• 期刊: Bioorg. Med. Chem. Lett. 19
• 作者: T. SAKAMOTO;M. INOUE;A. OJIDA;I. HAMACHI
• 通讯作者: I. HAMACHI