Developments of Fluorescent Probes for Elucidation of Biological Functions of Phosphate Species

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In this research project, we have developed the new fluorescent probes, which are useful for elucidation of biological functions of various biological phosphate species. We especially focused on the developments of the three types of the fluorescent probes, including 1) protein-based hybrid-type biosensor for phosphorylated protein/peptide, 2) molecular probe for aggregation of phosphorylated tau protein exisiting in brain of Alztheimer's disease, and 3) molecular probe for intracellular ATP with a OFF/ON-type fluorescence sensing mode. As the results, we have successfully achieved the probes with the desirable functions in each of the three themes.