Magnetic Beads Amplification based Biosensing System

基于磁珠扩增的生物传感系统

• 批准号: 22360139
• 负责人: SANDHU ADARSH
• 金额: $ 11.73万
• 依托单位: Toyohashi University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22360139/
• 关键词: 磁性粒子; 医療診断; ホール素子; バイオセンサー; 磁性粒子メーカー; ホール素子バイオセンサー; バイオセンサ; 磁性粒子マーカー; 蛍光マーカー; ホール素子バイオセンサ; 磁気増幅ビーズ; 高速な診断法; 標的磁性ビーズ; 標的ナノ磁性ビーズ

Detection of small concentrations of sub-200 nm sized SPBs (superparamagnetic beads) that are used as ‘magnetic labels’ is critical for rapid, highly sensitive, and portable point of care treatment (POCT). Currently, magnetoresistive (MR) biosensors are used for the detection of large concentrations of SPBs but such an approach is not suitable for monitoring small numbers of sub-200nm SPBs due to the intrinsic noise of these electronic devices. In order to overcome this limitation of conventional MR sensors, this research has led to the development of a straightforward procedure for detecting small concentrations of sub-200-nm-diameter SPBs for biosensing by exploiting magnetically induced self-assembly of micrometer sized SPBs onto nanometer targets. This approach enables the monitoring the capture of magnetically induced self-assembly of micrometer sized SPBs by nanometer magnetic beads under an optical microscope, and the physical amplification of the signal from otherwise undetectable nano-SPB targets using Hall biosensors without using the application of ac magnetic fields or lock-in detection, thereby enabling the production of a simple POCT protocol for a wide range of applications.

检测用作“磁性标签”的小浓度的小于200 nm尺寸的SPB（超顺磁珠）对于快速、高灵敏度和便携式护理点治疗（POCT）至关重要。目前，磁阻（MR）生物传感器用于检测大浓度的SPB，但是由于这些电子设备的固有噪声，这种方法不适合于监测少量的亚200nm SPB。为了克服传统MR传感器的这种局限性，本研究已经导致了一个简单的程序的开发，用于检测小浓度的子200 nm直径的SPBs的生物传感，通过利用磁诱导自组装的微米尺寸的SPBs到纳米目标。这种方法能够在光学显微镜下监测纳米磁珠对微米尺寸SPB的磁诱导自组装的捕获，以及使用霍尔生物传感器对来自原本不可检测的纳米SPB目标的信号的物理放大，而不使用交流磁场或锁定检测的应用，从而能够产生用于广泛应用的简单POCT协议。

项目成果

Adarsh Sandhu Detection of Magnetic Nanoparticles by Magnetically Mediated Self-Assembly of Superparamagnetic Microbeads for Biosensing

Adarsh Sandhu 通过用于生物传感的超顺磁性微珠的磁介导自组装来检测磁性纳米粒子

• 发表时间: 2009
• 作者: 森本義謙;阿部正紀;半田宏
• 通讯作者: 半田宏

Planar Microfluidic System based on Electrophoresis for Detection of 130-nm Magnetic Labels for Biosensing

基于电泳的平面微流体系统，用于检测生物传感中的 130 nm 磁性标记

• 发表时间: 2011
• 期刊: Japanese Journal of Applied Physics (JJAP)
• 作者: Naohiro Horiuchi;Takuya Hoshina;Hiroaki Takeda;Takaaki Tsurumi;Hayato Sano;堀内尚紘;佐野勇人;Naohiro Horiuchi;Hayato Sano;Naohiro Horiuchi;佐野勇人;Hayato Sano;Naohiro Horiuchi;Hayato Sano;Tomoko Matsumoto;Tomoko Matsumoto;村松綾;村松綾;村松綾;村松綾;村松綾;村松綾;Yoshitaka Morimoto;Tsukasa Takamura
• 通讯作者: Tsukasa Takamura

Detection of 8 nm diameter superparamagnetic beads by magnetically-induced manipulation of micrometer-sized magnetic beads: A novel protocol for magnetically-labeled biosensing

通过微米级磁珠的磁感应操作检测 8 nm 直径的超顺磁珠：磁标记生物传感的新方案

• 发表时间: 2010
• 期刊: Japanese Journal of Applied Physics
• 影响因子: 1.5
• 作者: Y. Morimoto,T. Takamura;S. Y. Park;S. Sakamoto;S. Kawata;H. Handa;and A Sandhu
• 通讯作者: and A Sandhu

Novel Protocol for Magnetically-Labeled Biosensing Platform

磁标记生物传感平台的新方案

• 发表时间: 2009
• 作者: A. Takeda;T. Aihara;M. Fukuhare. Y. Ishii. and M. Fukuda;Yoshitaka Morimoto and Adarsh Sandhu
• 通讯作者: Yoshitaka Morimoto and Adarsh Sandhu

Optical Transmittance for Analysis of the Dynamics of Self-Assembled Rotating Chains of Superparamagnetic Micro- and Nano Beads in Solution

光学透射率用于分析溶液中超顺磁微纳米珠自组装旋转链的动力学

• DOI: 10.1088/1742-6596/352/1/012002
• 发表时间: 2012
• 期刊: Journal of Physics Conf. Ser.
• 作者: T.Fukushima;K.-W.Lee;T.Tanaka;M.Koyanagi;M. Natsui;Pil Ju Ko
• 通讯作者: Pil Ju Ko