Studies on development and stabilization of intact liposome biosensor technology for Si-integrated structure

为了提高和稳定脂质体生物传感器及其传感技术的精度和性能，对作为未来硅集成微传感器系统关键部件的传感器进行了一系列技术考虑和改进。其技术发展始于脂质体悬浮液的微小液滴的固定化和完整的超分子结构。此外，基于电子学技术，开发了脂质体阻抗生物传感器和荧光阵列传感器等新型生物传感系统。最后，漏电流传感器和悬臂应变传感器的灵敏度和性能都得到了改善，因为微小脂质体液滴在传感器上的固定的定量能力得到了改善。我们还注意到，阻抗传感器可以检测100 nL量级的脂质体和靶蛋白之间的相互作用。并且荧光阵列传感器系统可以区分不同的目标生物分子。

In order to improve and stabilize the precision and performance of liposome biosensor and its sensing technologies, a series of technical considerations and resultant improvements have been done on the sensor as a key component of near future Si-integrated microsensor system. The technical developments are started from immobilization of minute droplet of liposome suspension and intact supramolecule structure. Moreover, new biosensing systems of liposome impedance biosensor and fluorescence array sensor are developed, based on electronics techniques. Finally, the sensitivities and performance of both leakage current sensor and cantilever strain sensor are improved because a resultant quantitative capability of immobilization of minute liposome droplet on the sensor becomes improved. We also note that the impedance sensor can detect the interaction between the liposome and target protein with a 100 nL order. And that the fluorescence array sensor system can discriminate different target biomolecules.