Efficient production of virus-like particles using insect cells

利用昆虫细胞高效生产病毒样颗粒

• 批准号: 22360345
• 负责人: YAMAJI Hideki
• 金额: $ 12.65万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22360345/
• 关键词: バイオ生産プロセス; 昆虫細胞; 組換えタンパク質生産; ウイルスタンパク質; ウイルス様粒子; ワクチン

Virus-like particles (VLPs) are composed of one or several recombinant viral surface proteins that spontaneously assemble into particulate structures similar to authentic virus particles or naturally occurring subviral particles. A high yield of a secretory form of flavivirus VLPs was successfully produced by lepidopteran insect cells engineered to coexpress the premembrane (prM) and envelope (E) proteins. This study demonstrated that recombinant insect cells offer a promising approach to the high-level production of VLPs for use as vaccines and diagnostic antigens.

病毒样颗粒(VLP)是由一个或几个重组病毒表面蛋白组成的，这些重组病毒表面蛋白自发组装成类似于正宗病毒颗粒或自然产生的亚病毒颗粒的颗粒结构。利用鳞翅目昆虫细胞共表达前膜蛋白(PRM)和外膜蛋白(E)，成功地获得了分泌型黄病毒VLPs。这项研究表明，重组昆虫细胞为高水平生产用作疫苗和诊断抗原的VLP提供了一种很有前途的方法。

项目成果

Evaluation of extracellular subviral particles of dengue type 2 virus produced by insect cells for use as vaccine and diagnostic antigens

昆虫细胞产生的登革热 2 型病毒胞外亚病毒颗粒用作疫苗和诊断抗原的评估

• 发表时间: 2011
• 作者: Miwa Kuwahara;Hideki Yamaji;Eiji Konishi
• 通讯作者: Eiji Konishi

Production and purification of Japanese encephalitis virus-like particles from recombinant insect cells

重组昆虫细胞制备和纯化日本脑炎病毒样颗粒

• 发表时间: 2012
• 作者: Yuri Hotta;Azusa Minamitani;Kazuma Narita;Tomohisa Katsuda;Hideki Yamaji
• 通讯作者: Hideki Yamaji

Efficient production of extracellular subviral particles of Japanese encephalitis virus by recombinant insect cells

重组昆虫细胞高效生产日本脑炎病毒胞外亚病毒颗粒

• 发表时间: 2011
• 作者: Hideki Yamaji;Takashi Nagasuga;Yusuke Takahashi;Masataka Nakamura;Tomohisa Katsuda;Miwa Kuwahara;Eiji Konishi
• 通讯作者: Eiji Konishi

Secretory production of virus-like particles from recombinant insect cells

从重组昆虫细胞分泌生产病毒样颗粒

• 发表时间: 2011
• 作者: Hideki Yamaji;Takashi Nagasuga;Yusuke Takahashi;Masataka Nakamura;Tomohisa Katsuda;Miwa Kuwahara;Eiji Konishi
• 通讯作者: Eiji Konishi

Production of Japanese encephalitis virus-like particles in insect cells

• DOI: 10.4161/bioe.24514
• 发表时间: 2013-05
• 期刊: Bioengineered
• 影响因子: 4.9
• 作者: H. Yamaji;E. Konishi