Production of apple sport by epigenetic variation system

利用整合了人工siRNA序列构建体的接穗，其siRNA分子通过韧皮部功能运输，诱导与siRNA具有互补序列的基因沉默(TGS：转录基因沉默)。在TGS的情况下，由于被调用的沉默通过DNA复制保持，TGS植株可以通过TGS组织的再生获得。本研究是为了将上述系统应用于营养作物苹果上而进行的分步试验。

Using the scion in which the construct producing an artificial siRNA sequence was integrated, its siRNA molecule transported through phloem functions to induce the silencing (TGS: transcriptional gene silencing) of the gene possessing complementary sequence with the siRNA. In case of TGS, as the invoked silencing maintains through DNA replication, the TGS plant can be obtained by the regeneration from the TGS tissue. This study was carried out the each step experiment in order to apply the above system to a vegetative crop, apple (Malus domestica).