Development of a simple gene delivery system into insects by lipofection

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The firefly luciferase gene was successfully expressed by in vivo lipofection in more than 70 species of insects belonging to six orders. Expression of the E22O gene encoding an ecdysteroid inactivating enzyme by lipofection effectively decreased the 20-hydroxyecdysone titer and blocked molting in B. mori. Combining this gene delivery system with the luciferase assay revealed that the silkworm paralytic peptide is encoded by a polycistronic mRNA. These results indicate that in vivo lipofection can be used to analyze functions or expression control mechanisms of genes in varieties of insects.